ID   ECCA1_MYCSE             Reviewed;         574 AA.
AC   L8FMA1; A0A2U9PH45;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=ESX-1 secretion system protein EccA1;
DE   AltName: Full=ESX conserved component A1;
DE   AltName: Full=Type VII secretion system protein EccA1 {ECO:0000305};
DE            Short=T7SS protein EccA1 {ECO:0000305};
GN   Name=eccA1 {ECO:0000305};
GN   ORFNames=0059 {ECO:0000303|PubMed:18554329},
GN   D806_000560 {ECO:0000312|EMBL:AWT51050.1}, D806_0059;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB
CC       (Probable). Plays a role in DNA conjugation, in both donor and
CC       recipient strains (PubMed:18554329). EccA1 exhibits ATPase activity and
CC       may provide energy for the export of ESX-1 substrates (By similarity).
CC       {ECO:0000250|UniProtKB:P9WPH9, ECO:0000269|PubMed:18554329}.
CC   -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components (By similarity).
CC       {ECO:0000250|UniProtKB:P9WPH9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU9}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain, strain does not secrete EsxB (PubMed:18554329).
CC       Increases efficiency of DNA conjugation when disrupted in donor strain
CC       (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
CC   -!- SIMILARITY: Belongs to the CbxX/CfxQ family. {ECO:0000305}.
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DR   EMBL; CP027541; AWT51050.1; -; Genomic_DNA.
DR   RefSeq; WP_003891386.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FMA1; -.
DR   SMR; L8FMA1; -.
DR   EnsemblBacteria; AWT51050; AWT51050; D806_000560.
DR   GeneID; 66738249; -.
DR   PATRIC; fig|1214915.3.peg.59; -.
DR   HOGENOM; CLU_008749_5_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   Gene3D; 1.25.40.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR041627; AAA_lid_6.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR000641; CbxX/CfxQ.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023835; T7SS_EccA.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF17866; AAA_lid_6; 1.
DR   PRINTS; PR00819; CBXCFQXSUPER.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03922; T7SS_EccA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Nucleotide-binding; Transport.
FT   CHAIN           1..574
FT                   /note="ESX-1 secretion system protein EccA1"
FT                   /id="PRO_0000438311"
FT   BINDING         335..342
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   574 AA;  62148 MW;  20482F23E84D753D CRC64;
     MVNHLAGMFG SAVGMMSNSR ARALEIFTEI TNYDESACDA WVGRIGCGDT DRVTLFRAWY
     SRSNFGQLAG AAEISMNGVG ARIPIGGIYS RDITYPINSP LAITMGFAVH EASEGNYTDA
     MEALEDAPAA GSEHLVSWVR AVIYGAGQRW TEVIDQVRGA ERWPDKFLAA AAGVAHGVAA
     ANLGLFTEAD RRLTEANDTP VAQACAPVIA WYLAMARRSQ GNEESAQVLL EWLQANFPEP
     KVTAALRDPA YRLETTTAEK IAARSDPWDP SSVVADTSAR DKLLVEAQAE LDRQIGLGRV
     KEQIEAYRAA TQMARIRAAR GMKVAQTSKH MIFAGPPGTG KTTIARVVAN ILAGLGVIAE
     PKLIETSRKD FVAEYEGQSA VKTSKTIDRA LGGVLFIDEA YTLVQERNGQ TDPFGAEALD
     TLLARMENDR DRLVVIIAGY SNDIDRLLEV NDGLRSRFAT RIEFDSYSPD EIVEISKVIA
     TANDSRLDDT AAKRVLEAAT TLSQRSLNGK PALDIAGNGR YARQLVEAGE QNRDMRLARS
     LDFDSLGVDQ LSEINGDDMA AAIASVHSRL NIGE