ID   ECCA3_MYCTO             Reviewed;         631 AA.
AC   P9WPI2; L0T677; O53687;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=ESX-3 secretion system protein EccA3 {ECO:0000250|UniProtKB:P9WPI3};
DE   AltName: Full=ESX conserved component A3 {ECO:0000250|UniProtKB:P9WPI3};
DE   AltName: Full=Type VII secretion system protein EccA3 {ECO:0000250|UniProtKB:P9WPI3};
DE            Short=T7SS protein EccA3 {ECO:0000250|UniProtKB:P9WPI3};
GN   Name=eccA3 {ECO:0000250|UniProtKB:P9WPI3}; OrderedLocusNames=MT0295;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Part of the ESX-3 specialized secretion system, which is
CC       important for iron and zinc uptake or homeostasis (By similarity).
CC       EccA3 exhibits ATPase activity and may provide energy for the export of
CC       ESX-3 substrates (By similarity). {ECO:0000250|UniProtKB:P9WPH9,
CC       ECO:0000250|UniProtKB:P9WPI3}.
CC   -!- SUBUNIT: Part of the ESX-3 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components.
CC       {ECO:0000250|UniProtKB:P9WPH9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU9}.
CC   -!- SIMILARITY: Belongs to the CbxX/CfxQ family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK44519.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK44519.1; ALT_INIT; Genomic_DNA.
DR   PIR; H70835; H70835.
DR   RefSeq; WP_003898392.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WPI2; -.
DR   SMR; P9WPI2; -.
DR   PRIDE; P9WPI2; -.
DR   EnsemblBacteria; AAK44519; AAK44519; MT0295.
DR   KEGG; mtc:MT0295; -.
DR   PATRIC; fig|83331.31.peg.318; -.
DR   HOGENOM; CLU_008749_5_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   Gene3D; 1.25.40.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR041627; AAA_lid_6.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR000641; CbxX/CfxQ.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR023835; T7SS_EccA.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF17866; AAA_lid_6; 1.
DR   PRINTS; PR00819; CBXCFQXSUPER.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03922; T7SS_EccA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Nucleotide-binding.
FT   CHAIN           1..631
FT                   /note="ESX-3 secretion system protein EccA3"
FT                   /id="PRO_0000426947"
FT   BINDING         385..392
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   631 AA;  68107 MW;  BE4D492E351C6C53 CRC64;
     MAGVGEGDSG GVERDDIGMV AASPVASRVN GKVDADVVGR FATCCRALGI AVYQRKRPPD
     LAAARSGFAA LTRVAHDQCD AWTGLAAAGD QSIGVLEAAS RTATTAGVLQ RQVELADNAL
     GFLYDTGLYL RFRATGPDDF HLAYAAALAS TGGPEEFAKA NHVVSGITER RAGWRAARWL
     AVVINYRAER WSDVVKLLTP MVNDPDLDEA FSHAAKITLG TALARLGMFA PALSYLEEPD
     GPVAVAAVDG ALAKALVLRA HVDEESASEV LQDLYAAHPE NEQVEQALSD TSFGIVTTTA
     GRIEARTDPW DPATEPGAED FVDPAAHERK AALLHEAELQ LAEFIGLDEV KRQVSRLKSS
     VAMELVRKQR GLTVAQRTHH LVFAGPPGTG KTTIARVVAK IYCGLGLLKR ENIREVHRAD
     LIGQHIGETE AKTNAIIDSA LDGVLFLDEA YALVATGAKN DFGLVAIDTL LARMENDRDR
     LVVIIAGYRA DLDKFLDTNE GLRSRFTRNI DFPSYTSHEL VEIAHKMAEQ RDSVFEQSAL
     HDLEALFAKL AAESTPDTNG ISRRSLDIAG NGRFVRNIVE RSEEEREFRL DHSEHAGSGE
     FSDEELMTIT ADDVGRSVEP LLRGLGLSVR A