ID   ECCB1_MYCSE             Reviewed;         479 AA.
AC   L8FM50; A0A2U9PH42;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=ESX-1 secretion system ATPase EccB1;
GN   ORFNames=0060 {ECO:0000303|PubMed:18554329},
GN   D806_000570 {ECO:0000312|EMBL:AWT51051.1}, D806_0060;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: An ATPase (By similarity). Part of the ESX-1 / type VII
CC       specialized secretion system (T7SS), which exports several proteins
CC       including EsxA and EsxB (Probable). Plays a role in DNA conjugation, in
CC       both donor and recipient strains (PubMed:18554329).
CC       {ECO:0000250|UniProtKB:A0QNJ0, ECO:0000269|PubMed:18554329,
CC       ECO:0000305}.
CC   -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. The ESX-1 membrane
CC       complex is composed of EccB1, EccCa1, EccCb1, EccD1 and EccE1 (By
CC       similarity). {ECO:0000250|UniProtKB:P9WNR7}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain, strain does not secrete EsxB (PubMed:18554329).
CC       {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
CC   -!- SIMILARITY: Belongs to the EccB family. {ECO:0000305}.
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DR   EMBL; CP027541; AWT51051.1; -; Genomic_DNA.
DR   RefSeq; WP_003891387.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FM50; -.
DR   SMR; L8FM50; -.
DR   EnsemblBacteria; AWT51051; AWT51051; D806_000570.
DR   PATRIC; fig|1214915.3.peg.60; -.
DR   HOGENOM; CLU_036302_3_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.910; -; 1.
DR   Gene3D; 3.30.2390.20; -; 1.
DR   InterPro; IPR007795; T7SS_EccB.
DR   InterPro; IPR044857; T7SS_EccB_R1.
DR   InterPro; IPR042485; T7SS_EccB_R3.
DR   PANTHER; PTHR40765; PTHR40765; 1.
DR   Pfam; PF05108; T7SS_ESX1_EccB; 1.
DR   TIGRFAMs; TIGR03919; T7SS_EccB; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Disulfide bond; Hydrolase;
KW   Membrane; Nucleotide-binding; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..479
FT                   /note="ESX-1 secretion system ATPase EccB1"
FT                   /id="PRO_0000438313"
FT   TOPO_DOM        1..44
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        66..479
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   DISULFID        152..347
FT                   /evidence="ECO:0000250|UniProtKB:A0QNJ0"
SQ   SEQUENCE   479 AA;  50995 MW;  4254A3CA3538040A CRC64;
     MAGFRLTTKV QVSGWRFLLR RVEHAIVRRD TRMFDDPLQF YSRAVFAGVV VSVLICLGAA
     LMAYFKPLGK QGSDQLLVDR TTNQLYVMLP GSNQLRPVYN LTSARLVLGN ASNPVAVKSE
     ELNRISKGQS IGIPGAPYAT PTGTPASQWT LCDTVAKPDS SAPKVETSIL IRTLAIDSGV
     GPIRADQGML VSYEGANWLI TEGGRHSIDL ADRAVTSAVG IPVTAKPTPI SQGLFNALPN
     RGPWQLPQIP AAGAPNSVGL PENLVIGSVF RTATESDPQH YVVLPDGVAR VNNTTAAALR
     ATNSYGLMQP PAVEASVVAK IPEQVYVSPL PDQPLDVLLR QDSPVLCWSW QREPGDQAPK
     TTVIAGRRLP LPASAIGTGI DQIGGDSTVY IEGGQFVRLQ SPDPRVGESM YYIDPQGVRY
     GIANDDAAKN LGLAGPVNAP WQVVGLLVDG PVLSKEAALI EHDTLPADPN PRKVASGEG