ECCC5_MYCMM
ID ECCC5_MYCMM Reviewed; 1388 AA.
AC B2HST4;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=ESX-5 secretion system protein EccC5 {ECO:0000305};
DE AltName: Full=ESX conserved component C5 {ECO:0000305};
DE AltName: Full=Type VII secretion system protein EccC5 {ECO:0000305};
DE Short=T7SS protein EccC5 {ECO:0000305};
GN Name=eccC5 {ECO:0000303|PubMed:22925462};
GN OrderedLocusNames=MMAR_2665 {ECO:0000312|EMBL:ACC41108.1};
OS Mycobacterium marinum (strain ATCC BAA-535 / M).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=216594;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-535 / M;
RX PubMed=18403782; DOI=10.1101/gr.075069.107;
RA Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K.,
RA Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C.,
RA Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K.,
RA Lord A., Moule S., Mungall K., Norbertczak H., Quail M.A.,
RA Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R.,
RA Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T.;
RT "Insights from the complete genome sequence of Mycobacterium marinum on the
RT evolution of Mycobacterium tuberculosis.";
RL Genome Res. 18:729-741(2008).
RN [2]
RP FUNCTION.
RX PubMed=19602152; DOI=10.1111/j.1365-2958.2009.06783.x;
RA Abdallah A.M., Verboom T., Weerdenburg E.M., Gey van Pittius N.C.,
RA Mahasha P.W., Jimenez C., Parra M., Cadieux N., Brennan M.J.,
RA Appelmelk B.J., Bitter W.;
RT "PPE and PE_PGRS proteins of Mycobacterium marinum are transported via the
RT type VII secretion system ESX-5.";
RL Mol. Microbiol. 73:329-340(2009).
RN [3]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-535 / M;
RX PubMed=22925462; DOI=10.1111/j.1365-2958.2012.08206.x;
RA Houben E.N., Bestebroer J., Ummels R., Wilson L., Piersma S.R.,
RA Jimenez C.R., Ottenhoff T.H., Luirink J., Bitter W.;
RT "Composition of the type VII secretion system membrane complex.";
RL Mol. Microbiol. 86:472-484(2012).
CC -!- FUNCTION: Part of the ESX-5 specialized secretion system, which is
CC responsible for the secretion of EsxN and a number of PE_PGRS and PPE
CC proteins (PubMed:19602152, PubMed:22925462). This component is
CC essential for ESX-5 complex stability and secretion (PubMed:22925462).
CC {ECO:0000269|PubMed:19602152, ECO:0000269|PubMed:22925462}.
CC -!- SUBUNIT: Part of the ESX-5 / type VII secretion system (T7SS), which is
CC composed of cytosolic and membrane components. The ESX-5 membrane
CC complex is composed of EccB5, EccC5, EccD5 and EccE5.
CC {ECO:0000269|PubMed:22925462}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:22925462}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Mutant does not secrete EsxN and PE_PGRS
CC proteins, and has reduced levels of EccB5, EccD5 and EccE5.
CC {ECO:0000269|PubMed:22925462}.
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DR EMBL; CP000854; ACC41108.1; -; Genomic_DNA.
DR RefSeq; WP_012394382.1; NC_010612.1.
DR AlphaFoldDB; B2HST4; -.
DR SMR; B2HST4; -.
DR STRING; 216594.MMAR_2665; -.
DR PRIDE; B2HST4; -.
DR EnsemblBacteria; ACC41108; ACC41108; MMAR_2665.
DR KEGG; mmi:MMAR_2665; -.
DR eggNOG; COG1674; Bacteria.
DR HOGENOM; CLU_003134_1_0_11; -.
DR OMA; QPSAQVW; -.
DR OrthoDB; 7548at2; -.
DR PHI-base; PHI:6277; -.
DR Proteomes; UP000001190; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 4.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR023836; EccCa-like_Actinobacteria.
DR InterPro; IPR023837; EccCb-like_Actinobacteria.
DR InterPro; IPR002543; FtsK_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01580; FtsK_SpoIIIE; 2.
DR SMART; SM00382; AAA; 3.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR03924; T7SS_EccC_a; 1.
DR TIGRFAMs; TIGR03925; T7SS_EccC_b; 1.
DR PROSITE; PS50901; FTSK; 3.
PE 1: Evidence at protein level;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..1388
FT /note="ESX-5 secretion system protein EccC5"
FT /id="PRO_0000434750"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 477..679
FT /note="FtsK 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT DOMAIN 855..1049
FT /note="FtsK 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT DOMAIN 1158..1351
FT /note="FtsK 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 500..507
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 873..880
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 1175..1182
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
SQ SEQUENCE 1388 AA; 152570 MW; 1BFC4383FBB63A31 CRC64;
MKRGFARPTP EKAPVIKPEN IVLPTPLSIP PPEGKPWWLI VVGVVVVGLL GGMVAMVFAS
GSHVFGGVGS IFPIFMMVGI MMMMFRSVGA GGQQQMSRPK LDAMRAQFML MLDMLRETAQ
ESADSMDSNY RWFHPAPSTL AAAVGSPRMW ERKPDGKDLN FGVVRVGVGM TRPEVTWGEP
QNMPTDIELE PVTGKALQEF GRYQSVVYNL PKMISLLVEP WYALVGEREQ ALGLMRAIIC
QLTFSHGPDH VQFIVVSSDL AEWEWVKWLP HFGDSRRYDA AGNARMVYSS VREFAAEQGE
LFAGRGSFTP RHASSSAQTP TPHTVIICDV DDPQWEYVIS AEGVDGVTFF DLTGSPMWTN
VPERKLEFDK TGVIEALPRD RDTWMVIDDN AWFFALTDHV SIAEAEEFGQ KLAQWRLAEA
YEEIGQRVAH IGARDILAYY GIDDPGNIDF DYLWGSRTDS MGRSRLRAPF GNRSDNGELL
FLDMKSLDEG GDGPHGVMSG TTGSGKSTLV RTVIESLMLG HPPEELQFVL ADLKGGSAVK
PFAGVPHVSR IITDLEEDQA LMERFLDALW GEIARRKAIC DSAGVDDAKE YNSVRGRMRA
RGQDMAPLPM LVVVIDEFYE WFRIMPTAVD VLDSIGRQGR AYWIHLMMAS QTIESRAEKL
MENMGYRLVL KARTAGAAQA AGVPNAVNLP AQAGLGYFRK SLEDIIRFQA EFLWRDYFQP
GITVDGEEAP VLVHSIDYIR PQLFTNSFTP LEVTVGGPEI DKVVAHANGE VVEEVEAEAE
EEGIRVPKVG TVIIDQLRRI NFEPYRLWQP PLTQPVAIDD LVNRFLGHPW QKEYGSARNL
VFPIGVIDRP FKHDQPPWTV DTSGPGSNVL ILGAGGSGKT TALQTLISSA ALTHTPDQVQ
FYCLAYSSTA LTTVSKLPHV GEVAGPTDPY GVRRTVAELL ALVRERKRSF LEYGIASMEM
FRRRKFGGEA GPVPNDGFGD VYLVIDNYRA LAEENEVLIE QVNLIINQGP SFGVHVVVTA
DRESELRPPV RSGFGSRVEL RLAAVEDAKL VRSRFAKDVP VKPGRGMVAV NYVRLDSDPQ
AGLHTLVARP AMGSTPTNVF ECDSVVAAVS RLTTSQAPPV RRLPASFGVD QVRQLAARDT
RQGVGVGGIA WAISELDLQP VYLNFAENSH LMVTGRRECG RTTTLATIMS EIGRLYAPGA
TSVPAPPPGQ PSAQVWLIDP RRQLLTALGS NYVERFAYNL DGVQAMMGEL AAVLAGREPP
PGLSAEELLS RSWWSGPEIF LIVDDIQQLP PGFDSPLHKA APWVNRAADV GLHVIVTRSF
GGWSSAGSDP MLRALHQANA PLLVMDADPD EGFIRGKMKG GPLPRGRGLL MAEDTGVFVQ
VALTEVRK
