ECCD3_MYCS2
ID ECCD3_MYCS2 Reviewed; 475 AA.
AC A0QQ46;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=ESX-3 secretion system protein EccD3 {ECO:0000250|UniProtKB:P9WNQ3};
DE AltName: Full=ESX conserved component D3 {ECO:0000250|UniProtKB:P9WNQ3};
DE AltName: Full=Type VII secretion system protein EccD3 {ECO:0000250|UniProtKB:P9WNQ3};
DE Short=T7SS protein EccD3 {ECO:0000250|UniProtKB:P9WNQ3};
GN Name=eccD3 {ECO:0000303|PubMed:24803520};
GN Synonyms=snm {ECO:0000312|EMBL:ABK74115.1};
GN OrderedLocusNames=MSMEG_0623 {ECO:0000312|EMBL:ABK74115.1},
GN MSMEI_0607 {ECO:0000312|EMBL:AFP37088.1};
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION.
RX PubMed=19846780; DOI=10.1073/pnas.0900589106;
RA Siegrist M.S., Unnikrishnan M., McConnell M.J., Borowsky M., Cheng T.Y.,
RA Siddiqi N., Fortune S.M., Moody D.B., Rubin E.J.;
RT "Mycobacterial Esx-3 is required for mycobactin-mediated iron
RT acquisition.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:18792-18797(2009).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24803520; DOI=10.1128/mbio.01073-14;
RA Siegrist M.S., Steigedal M., Ahmad R., Mehra A., Dragset M.S.,
RA Schuster B.M., Philips J.A., Carr S.A., Rubin E.J.;
RT "Mycobacterial Esx-3 requires multiple components for iron acquisition.";
RL MBio 5:E01073-E01073(2014).
CC -!- FUNCTION: Part of the ESX-3 specialized secretion system, which is
CC required for siderophore-mediated iron acquisition and for the
CC secretion of EsxH and EsxG. {ECO:0000269|PubMed:19846780,
CC ECO:0000269|PubMed:24803520}.
CC -!- SUBUNIT: Part of the ESX-3 / type VII secretion system (T7SS), which is
CC composed of cytosolic and membrane components. The ESX-3 membrane
CC complex is composed of EccB3, EccC3, EccD3 and EccE3.
CC {ECO:0000250|UniProtKB:B2HSU6}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:B2HSU6}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene impairs iron-bound
CC mycobactin utilization and EsxG and EsxH export.
CC {ECO:0000269|PubMed:24803520}.
CC -!- SIMILARITY: Belongs to the EccD/Snm4 family. {ECO:0000305}.
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DR EMBL; CP000480; ABK74115.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP37088.1; -; Genomic_DNA.
DR RefSeq; WP_011727073.1; NZ_SIJM01000009.1.
DR RefSeq; YP_885034.1; NC_008596.1.
DR PDB; 6LAR; EM; 3.70 A; B/C/E/H=1-475.
DR PDB; 6SGW; EM; 3.80 A; B/C/E/H=8-472.
DR PDB; 6SGX; EM; 3.70 A; B/C=8-472.
DR PDB; 6SGZ; EM; 3.90 A; E/H=6-472.
DR PDB; 6UMM; EM; 3.70 A; B/C/G/H=1-475.
DR PDBsum; 6LAR; -.
DR PDBsum; 6SGW; -.
DR PDBsum; 6SGX; -.
DR PDBsum; 6SGZ; -.
DR PDBsum; 6UMM; -.
DR AlphaFoldDB; A0QQ46; -.
DR SMR; A0QQ46; -.
DR STRING; 246196.MSMEI_0607; -.
DR PRIDE; A0QQ46; -.
DR EnsemblBacteria; ABK74115; ABK74115; MSMEG_0623.
DR EnsemblBacteria; AFP37088; AFP37088; MSMEI_0607.
DR GeneID; 66738801; -.
DR KEGG; msg:MSMEI_0607; -.
DR KEGG; msm:MSMEG_0623; -.
DR PATRIC; fig|246196.19.peg.619; -.
DR eggNOG; ENOG5031ZT8; Bacteria.
DR OMA; WDSAPCK; -.
DR OrthoDB; 1102071at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR006707; T7SS_EccD.
DR InterPro; IPR024962; YukD-like.
DR Pfam; PF08817; YukD; 1.
DR PIRSF; PIRSF017804; Secretion_EccD1; 1.
DR TIGRFAMs; TIGR03920; T7SS_EccD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..475
FT /note="ESX-3 secretion system protein EccD3"
FT /id="PRO_0000434992"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 475 AA; 48261 MW; 7D648570E2364DC2 CRC64;
MSENTVMPIV RVAVLAAGDD GGRLTEMALP SELPLREILP AVQRIVQPAR ENDGAADPAA
APNPVRLSLA PIGGAPFSLD ATLDTVGVVD GDLLALQAVP SGPPAPRIVE DIADAAVIFS
EARRRQWGPT HIARGAALAL IGLILVGTGL SVAHRVITGD LLGQFIVSGI ALATVIAALA
VRNRSAVLAT SLAVTALVPV AAAFALGVPG DFGAPNVLLA AAGVAAWSLI SMAGSPDDRG
IAVFTATAVT GVGVLLVAGA ASLWVISSDV IGCALVLLGL IVTVQAAQLS AMWARFPLPV
IPAPGDPTPA ARPLSVLADL PRRVRVSQAH QTGVIAAGVL LGVAGSVALV SSANASPWAW
YIVVAAAAGA ALRARVWDSA ACKAWLLGHS YLLAVALLVA FVIGDRYQAA LWALAALAVL
VLVWIVAALN PKIASPDTYS LPMRRMVGFL ATGLDASLIP VMALLVGLFS LVLDR
