ID   ECCD4_MYCTU             Reviewed;         467 AA.
AC   P9WNQ1; L0TFN3; O33354; Q7D5I9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=ESX-4 secretion system protein eccD4;
DE   AltName: Full=ESX conserved component D4;
DE   AltName: Full=Type VII secretion system protein eccD4;
DE            Short=T7SS protein eccD4;
GN   Name=eccD4; OrderedLocusNames=Rv3448;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   GENE NAME.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
CC   -!- SUBUNIT: Part of the ESX-4 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the EccD/Snm4 family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP46270.1; -; Genomic_DNA.
DR   PIR; E70564; E70564.
DR   RefSeq; NP_217965.1; NC_000962.3.
DR   RefSeq; WP_003900062.1; NZ_NVQJ01000065.1.
DR   AlphaFoldDB; P9WNQ1; -.
DR   SMR; P9WNQ1; -.
DR   STRING; 83332.Rv3448; -.
DR   PaxDb; P9WNQ1; -.
DR   DNASU; 887624; -.
DR   GeneID; 887624; -.
DR   KEGG; mtu:Rv3448; -.
DR   TubercuList; Rv3448; -.
DR   eggNOG; ENOG502ZAY5; Bacteria.
DR   OMA; LRARTHC; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR044049; EccD_transm.
DR   InterPro; IPR006707; T7SS_EccD.
DR   InterPro; IPR024962; YukD-like.
DR   Pfam; PF19053; EccD; 1.
DR   Pfam; PF08817; YukD; 1.
DR   PIRSF; PIRSF017804; Secretion_EccD1; 1.
DR   TIGRFAMs; TIGR03920; T7SS_EccD; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..467
FT                   /note="ESX-4 secretion system protein eccD4"
FT                   /id="PRO_0000393236"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        319..339
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        374..394
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        401..421
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        439..459
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   467 AA;  46731 MW;  91FF9CD1FD51F2F4 CRC64;
     MPTSDPGLRR VTVHAGAQAV DLTLPAAVPV ATLIPSIVDI LGDRGASPAT AARYQLSALG
     APALPNATTL AQCGIRDGAV LVLHKSSAQP PTPRCDDVAE AVAAALDTTA RPQCQRTTRL
     SGALAASCIT AGGGLMLVRN ALGTNVTRYS DATAGVVAAA GLAALLFAVI ACRTYRDPIA
     GLTLSVIATI FGAVAGLLAV PGVPGVHSVL VAAMAAAATS VLAMRITGCG GITLTAVACC
     AVVVAAATLV GAITAAPVPA IGSLATLASF GLLEVSARMA VLLAGLSPRL PPALNPDDAD
     ALPTTDRLTT RANRADAWLT SLLAAFAASA TIGAIGTAVA THGIHRSSMG GIALAAVTGA
     LLLLRARSAD TRRSLVFAIC GITTVATAFT VAADRALEHG PWIAALTAML AAVAMFLGFV
     APALSLSPVT YRTIELLECL ALIAMVPLTA WLCGAYSAVR HLDLTWT