ID   ECCE1_MYCS2             Reviewed;         464 AA.
AC   A0QNL0;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=ESX-1 secretion system protein EccE1;
GN   Name=eccE1 {ECO:0000303|PubMed:19876390};
GN   Synonyms=Ms3382c {ECO:0000303|PubMed:15314236},
GN   Sm3882c {ECO:0000303|PubMed:15687187}, snm7 {ECO:0000303|PubMed:15687187};
GN   OrderedLocusNames=MSMEG_0082, MSMEI_0080;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=15314236; DOI=10.1073/pnas.0404892101;
RA   Flint J.L., Kowalski J.C., Karnati P.K., Derbyshire K.M.;
RT   "The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA
RT   transfer in Mycobacterium smegmatis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12598-12603(2004).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=15687187; DOI=10.1128/jb.187.4.1238-1245.2005;
RA   Converse S.E., Cox J.S.;
RT   "A protein secretion pathway critical for Mycobacterium tuberculosis
RT   virulence is conserved and functional in Mycobacterium smegmatis.";
RL   J. Bacteriol. 187:1238-1245(2005).
RN   [6]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB
CC       (PubMed:15687187). Plays a role in DNA conjugation, in at least a donor
CC       strain (PubMed:15314236). {ECO:0000269|PubMed:15314236,
CC       ECO:0000269|PubMed:15687187}.
CC   -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. The ESX-1 membrane
CC       complex is composed of EccB1, EccCa1, EccCb1, EccD1 and EccE1 (By
CC       similarity). {ECO:0000250|UniProtKB:P9WJE9,
CC       ECO:0000305|PubMed:19876390}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Increases efficiency of DNA conjugation when
CC       disrupted in donor strain (PubMed:15314236). Loss of secretion of EsxA
CC       and EsxB, but not their expression (PubMed:15687187).
CC       {ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:15687187}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:15314236}.
CC   -!- SIMILARITY: Belongs to the EccE family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK70621.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36562.1; -; Genomic_DNA.
DR   RefSeq; WP_011726653.1; NZ_SIJM01000048.1.
DR   RefSeq; YP_884498.1; NC_008596.1.
DR   AlphaFoldDB; A0QNL0; -.
DR   STRING; 246196.MSMEI_0080; -.
DR   PRIDE; A0QNL0; -.
DR   EnsemblBacteria; ABK70621; ABK70621; MSMEG_0082.
DR   EnsemblBacteria; AFP36562; AFP36562; MSMEI_0080.
DR   GeneID; 66738273; -.
DR   KEGG; msg:MSMEI_0080; -.
DR   KEGG; msm:MSMEG_0082; -.
DR   PATRIC; fig|246196.19.peg.80; -.
DR   eggNOG; ENOG5031ZQC; Bacteria.
DR   OMA; RTWIMLR; -.
DR   OrthoDB; 383267at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR021368; T7SS_EccE.
DR   Pfam; PF11203; EccE; 1.
DR   TIGRFAMs; TIGR03923; T7SS_EccE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..464
FT                   /note="ESX-1 secretion system protein EccE1"
FT                   /id="PRO_0000438350"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   464 AA;  51023 MW;  0D46E52940406076 CRC64;
     MNFLRQFGFR FTTGHGIWAA TLIPACIAIC MHFDLLWLGI TLGALIALFS VLTIRGLRIT
     GWVRAIFSWR RRHRSTPDIA SEPAVGSTVM PGDHVAVRWQ GDYLIAVIEL VPRPFTPTVI
     VNGSAMTDDV VNTKLVERLL EAHCPDLEAD VVSAGYRVGK TAPASLIALY EQVVGPYPAP
     ANRRTWIVLR ADPEKTQRSA HRRDSGVSGL ARYLVASATR IADQLASNGV DARCCRSFDD
     YEKATEISYE RETWSSIKGR STFTAAYTAP GGPDMWWSAR ADHTITRVRV RPGAAPSSAI
     LLTTLANPTT PRGFSCLFGG QRAALQGIVP VSDKHYDLPI GSAGVLVGET ADRYPVYMPF
     DNVDVSINLG DARLFTQFVI RSAAAGAVVT LGPQFREFAT MINGRVGRTP RIGWPNATTY
     LGPHQGVGRV ILRPNFIDTP RHRQLPIQLI NPREESRYQM ALEQ