ID   ECCE3_MYCS2             Reviewed;         309 AA.
AC   A0QQ48;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=ESX-3 secretion system protein EccE3 {ECO:0000250|UniProtKB:P9WJE5};
DE   AltName: Full=ESX conserved component E3 {ECO:0000250|UniProtKB:P9WJE5};
DE   AltName: Full=Type VII secretion system protein EccE3 {ECO:0000250|UniProtKB:P9WJE5};
DE            Short=T7SS protein EccE3 {ECO:0000250|UniProtKB:P9WJE5};
GN   Name=eccE3 {ECO:0000303|PubMed:24803520};
GN   OrderedLocusNames=MSMEG_0626 {ECO:0000312|EMBL:ABK73896.1},
GN   MSMEI_0609 {ECO:0000312|EMBL:AFP37090.1};
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION.
RX   PubMed=19846780; DOI=10.1073/pnas.0900589106;
RA   Siegrist M.S., Unnikrishnan M., McConnell M.J., Borowsky M., Cheng T.Y.,
RA   Siddiqi N., Fortune S.M., Moody D.B., Rubin E.J.;
RT   "Mycobacterial Esx-3 is required for mycobactin-mediated iron
RT   acquisition.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:18792-18797(2009).
RN   [5]
RP   FUNCTION.
RX   PubMed=24803520; DOI=10.1128/mbio.01073-14;
RA   Siegrist M.S., Steigedal M., Ahmad R., Mehra A., Dragset M.S.,
RA   Schuster B.M., Philips J.A., Carr S.A., Rubin E.J.;
RT   "Mycobacterial Esx-3 requires multiple components for iron acquisition.";
RL   MBio 5:E01073-E01073(2014).
CC   -!- FUNCTION: Part of the ESX-3 specialized secretion system, which is
CC       required for siderophore-mediated iron acquisition and for the
CC       secretion of EsxH and EsxG. {ECO:0000269|PubMed:19846780,
CC       ECO:0000269|PubMed:24803520}.
CC   -!- SUBUNIT: Part of the ESX-3 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. The ESX-3 membrane
CC       complex is composed of EccB3, EccC3, EccD3 and EccE3.
CC       {ECO:0000250|UniProtKB:B2HSU8}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:B2HSU8}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the EccE family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK73896.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP37090.1; -; Genomic_DNA.
DR   RefSeq; WP_003892045.1; NZ_SIJM01000009.1.
DR   RefSeq; YP_885036.1; NC_008596.1.
DR   PDB; 6LAR; EM; 3.70 A; D/G=1-309.
DR   PDB; 6SGW; EM; 3.80 A; D/G=1-285.
DR   PDB; 6SGX; EM; 3.70 A; G=1-285.
DR   PDB; 6SGZ; EM; 3.90 A; D=2-287.
DR   PDB; 6UMM; EM; 3.70 A; A/F=1-285.
DR   PDBsum; 6LAR; -.
DR   PDBsum; 6SGW; -.
DR   PDBsum; 6SGX; -.
DR   PDBsum; 6SGZ; -.
DR   PDBsum; 6UMM; -.
DR   AlphaFoldDB; A0QQ48; -.
DR   SMR; A0QQ48; -.
DR   STRING; 246196.MSMEI_0609; -.
DR   PRIDE; A0QQ48; -.
DR   EnsemblBacteria; ABK73896; ABK73896; MSMEG_0626.
DR   EnsemblBacteria; AFP37090; AFP37090; MSMEI_0609.
DR   GeneID; 66738803; -.
DR   KEGG; msg:MSMEI_0609; -.
DR   KEGG; msm:MSMEG_0626; -.
DR   PATRIC; fig|246196.19.peg.621; -.
DR   eggNOG; ENOG5031E1N; Bacteria.
DR   OMA; MAYPWHS; -.
DR   OrthoDB; 1399338at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR021368; T7SS_EccE.
DR   Pfam; PF11203; EccE; 1.
DR   TIGRFAMs; TIGR03923; T7SS_EccE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..309
FT                   /note="ESX-3 secretion system protein EccE3"
FT                   /id="PRO_0000434993"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   309 AA;  33185 MW;  BDD4ADD314E81F8C CRC64;
     MTARIALASL FVVAAVLAQP WQTTTQRWVL GVSIAAVIVL LAWWKGMFLT TRIGRALAMV
     RRNRAEDTVE TDAHRATVVL RVDPAAPAQL PVVVGYLDRY GITCDKVRIT HRDAGGTRRS
     WISLTVDAVD NLAALQARSA RIPLQDTTEV VGRRLADHLR EQGWTVTVVE GVDTPLPVSG
     KETWRGVADD AGVVAAYRVK VDDRLDEVLA EIGHLPAEET WTALEFTGSP AEPLLTVCAA
     VRTSDRPAAK APLAGLTPAR GRHRPALAAL NPLSTERLDG TAVPLPAVVR TSVKGSVEHE
     AAQEAGHPA