ID   ECCE3_MYCTU             Reviewed;         331 AA.
AC   P9WJE5; L0T688; O53696; Q7DA30;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=ESX-3 secretion system protein EccE3 {ECO:0000305};
DE   AltName: Full=ESX conserved component E3 {ECO:0000305};
DE   AltName: Full=Type VII secretion system protein EccE3 {ECO:0000305};
DE            Short=T7SS protein EccE3 {ECO:0000305};
GN   Name=eccE3 {ECO:0000303|PubMed:19876390}; OrderedLocusNames=Rv0292;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INDUCTION.
RX   PubMed=12065475; DOI=10.1128/iai.70.7.3371-3381.2002;
RA   Rodriguez G.M., Voskuil M.I., Gold B., Schoolnik G.K., Smith I.;
RT   "IdeR, an essential gene in Mycobacterium tuberculosis: role of IdeR in
RT   iron-dependent gene expression, iron metabolism, and oxidative stress
RT   response.";
RL   Infect. Immun. 70:3371-3381(2002).
RN   [3]
RP   INDUCTION.
RX   PubMed=17098899; DOI=10.1128/jb.01190-06;
RA   Maciag A., Dainese E., Rodriguez G.M., Milano A., Provvedi R., Pasca M.R.,
RA   Smith I., Palu G., Riccardi G., Manganelli R.;
RT   "Global analysis of the Mycobacterium tuberculosis Zur (FurB) regulon.";
RL   J. Bacteriol. 189:730-740(2007).
RN   [4]
RP   FUNCTION.
RC   STRAIN=H37Rv;
RX   PubMed=19684129; DOI=10.1128/jb.00756-09;
RA   Serafini A., Boldrin F., Palu G., Manganelli R.;
RT   "Characterization of a Mycobacterium tuberculosis ESX-3 conditional mutant:
RT   essentiality and rescue by iron and zinc.";
RL   J. Bacteriol. 191:6340-6344(2009).
RN   [5]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [7]
RP   FUNCTION.
RX   PubMed=24155985; DOI=10.1371/journal.pone.0078351;
RA   Serafini A., Pisu D., Palu G., Rodriguez G.M., Manganelli R.;
RT   "The ESX-3 secretion system is necessary for iron and zinc homeostasis in
RT   Mycobacterium tuberculosis.";
RL   PLoS ONE 8:E78351-E78351(2013).
CC   -!- FUNCTION: Part of the ESX-3 specialized secretion system, which is
CC       important for iron and zinc uptake or homeostasis.
CC       {ECO:0000269|PubMed:19684129, ECO:0000269|PubMed:24155985}.
CC   -!- SUBUNIT: Part of the ESX-3 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. The ESX-3 membrane
CC       complex is composed of EccB3, EccC3, EccD3 and EccE3.
CC       {ECO:0000250|UniProtKB:B2HSU8}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:B2HSU8}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Repressed by IdeR in the presence of iron and by Zur in the
CC       presence of zinc. {ECO:0000269|PubMed:12065475,
CC       ECO:0000269|PubMed:17098899}.
CC   -!- SIMILARITY: Belongs to the EccE family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP43022.1; -; Genomic_DNA.
DR   PIR; B70837; B70837.
DR   RefSeq; NP_214806.1; NC_000962.3.
DR   RefSeq; WP_003916638.1; NZ_NVQJ01000026.1.
DR   AlphaFoldDB; P9WJE5; -.
DR   SMR; P9WJE5; -.
DR   STRING; 83332.Rv0292; -.
DR   PaxDb; P9WJE5; -.
DR   DNASU; 886601; -.
DR   GeneID; 886601; -.
DR   KEGG; mtu:Rv0292; -.
DR   TubercuList; Rv0292; -.
DR   eggNOG; ENOG5031E1N; Bacteria.
DR   OMA; MAYPWHS; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   InterPro; IPR021368; T7SS_EccE.
DR   Pfam; PF11203; EccE; 1.
DR   TIGRFAMs; TIGR03923; T7SS_EccE; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..331
FT                   /note="ESX-3 secretion system protein EccE3"
FT                   /id="PRO_0000393871"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   331 AA;  35932 MW;  E39848266F4682B7 CRC64;
     MNPIPSWPGR GRVTLVLLAV VPVALAYPWQ STRDYVLLGV AAAVVIGLFG FWRGLYFTTI
     ARRGLAILRR RRRIAEPATC TRTTVLVWVG PPASDTNVLP LTLIARYLDR YGIRADTIRI
     TSRVTASGDC RTWVGLTVVA DDNLAALQAR SARIPLQETA QVAARRLADH LREIGWEAGT
     AAPDEIPALV AADSRETWRG MRHTDSDYVA AYRVSANAEL PDTLPAIRSR PAQETWIALE
     IAYAAGSSTR YTVAAACALR TDWRPGGTAP VAGLLPQHGN HVPALTALDP RSTRRLDGHT
     DAPADLLTRL HWPTPTAGAH RAPLTNAVSR T