ID   ECDF_ASPRU              Reviewed;         508 AA.
AC   K0E681;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Putative glycosyl hydrolase ecdF {ECO:0000303|PubMed:22998630};
DE            EC=3.2.1.- {ECO:0000305|PubMed:22998630};
DE   Flags: Precursor;
GN   Name=ecdF {ECO:0000303|PubMed:22998630};
OS   Aspergillus rugulosus (Emericella rugulosa).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=41736;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 58397 / NRRL 11440;
RX   PubMed=22998630; DOI=10.1021/ja307220z;
RA   Cacho R.A., Jiang W., Chooi Y.H., Walsh C.T., Tang Y.;
RT   "Identification and characterization of the echinocandin B biosynthetic
RT   gene cluster from Emericella rugulosa NRRL 11440.";
RL   J. Am. Chem. Soc. 134:16781-16790(2012).
RN   [2]
RP   CLUSTER REVISION.
RX   PubMed=27502607; DOI=10.1186/s12864-016-2885-x;
RA   Huettel W., Youssar L., Gruening B.A., Guenther S., Hugentobler K.G.;
RT   "Echinocandin B biosynthesis: a biosynthetic cluster from Aspergillus
RT   nidulans NRRL 8112 and reassembly of the subclusters Ecd and Hty from
RT   Aspergillus pachycristatus NRRL 11440 reveals a single coherent gene
RT   cluster.";
RL   BMC Genomics 17:570-570(2016).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
CC   -!- CAUTION: EcdB, ecdC, ecdD, ecdE and ecdF have previously been
CC       identified as being part of the echinocandin B biosynthetic cluster,
CC       but it was later realized that this was due to a genome misassembly and
CC       these 5 proteins are now considered as artifacts and not part of the
CC       cluster. {ECO:0000269|PubMed:27502607}.
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DR   EMBL; JX421684; AFT91384.1; -; Genomic_DNA.
DR   AlphaFoldDB; K0E681; -.
DR   SMR; K0E681; -.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR001362; Glyco_hydro_32.
DR   InterPro; IPR013189; Glyco_hydro_32_C.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF08244; Glyco_hydro_32C; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SMART; SM00640; Glyco_32; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..508
FT                   /note="Putative glycosyl hydrolase ecdF"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5013130535"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        122
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        275
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        362
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   508 AA;  54821 MW;  24E4E153F8819E6A CRC64;
     MFLHILCLLA GQALADDYRP VFHFVPEKNW MNEPNGLIKI GSTWHLFYQH NPTANVWGNL
     NWGHATSSDL VHWTHEPLAI TSENGIEAFT GTSYYDAENT SGLGTSDNPP YLAWYTGYFP
     SNGTQDQRLA FSIDAGETWT KFEGNPVISA AQEAPHDATG GLETRDPKVF FHADSGKWIM
     VLAHGGQNKM SFWTSVDAKR WSWASDLTST QVPGLPSAVK GWEVPDMFEV PVHGTDKTTW
     VLIFTPAEGS PAGGNGVLAL TGSFDGTVFH PNPVNVSTLW LDYGRDFDGA LSWENLPDSD
     GHRILAAISN SYGANPPTNT WKGMLSFPRT LSLHQSQTGQ YFLQQPVSNL DTVSTPLVSV
     KNQTIAPGQV LLSSVRGTAL DIRIAFSANA GTVLSLAVRK AGSQETVIQY RQSDATLSVD
     RTTSGLTSYD PAAGGVHTAP LRPDASGVVQ IRALVDTCSV EVFGGQGEVV ISDLIFPDET
     SDGLALQVIG GTAVLRSLEK FPRLGAVL