ECD_DROME
ID ECD_DROME Reviewed; 684 AA.
AC Q9W032;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein ecdysoneless {ECO:0000305|PubMed:15128659};
GN Name=ecd {ECO:0000312|FlyBase:FBgn0000543}; ORFNames=CG5714;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND MUTAGENESIS OF PRO-656.
RX PubMed=15128659; DOI=10.1242/dev.01143;
RA Gaziova I., Bonnette P.C., Henrich V.C., Jindra M.;
RT "Cell-autonomous roles of the ecdysoneless gene in Drosophila development
RT and oogenesis.";
RL Development 131:2715-2725(2004).
CC -!- FUNCTION: Required in both the follicle cells and the germline for
CC oocyte development. {ECO:0000269|PubMed:15128659}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15128659}.
CC -!- TISSUE SPECIFICITY: Expressed in the ecdysone-producing larval ring
CC gland, nervous system, imaginal disks and gonads.
CC {ECO:0000269|PubMed:15128659}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development. {ECO:0000269|PubMed:15128659}.
CC -!- SIMILARITY: Belongs to the ECD family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF47628.1; -; Genomic_DNA.
DR EMBL; AY051483; AAK92907.1; -; mRNA.
DR RefSeq; NP_647707.1; NM_139450.3.
DR AlphaFoldDB; Q9W032; -.
DR BioGRID; 63806; 21.
DR IntAct; Q9W032; 6.
DR STRING; 7227.FBpp0072781; -.
DR PaxDb; Q9W032; -.
DR PRIDE; Q9W032; -.
DR DNASU; 38291; -.
DR EnsemblMetazoa; FBtr0072903; FBpp0072781; FBgn0000543.
DR GeneID; 38291; -.
DR KEGG; dme:Dmel_CG5714; -.
DR UCSC; CG5714-RA; d. melanogaster.
DR CTD; 11319; -.
DR FlyBase; FBgn0000543; ecd.
DR VEuPathDB; VectorBase:FBgn0000543; -.
DR eggNOG; KOG2406; Eukaryota.
DR GeneTree; ENSGT00390000015361; -.
DR HOGENOM; CLU_006241_2_0_1; -.
DR InParanoid; Q9W032; -.
DR OMA; TKDYIWQ; -.
DR OrthoDB; 603336at2759; -.
DR PhylomeDB; Q9W032; -.
DR BioGRID-ORCS; 38291; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38291; -.
DR PRO; PR:Q9W032; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000543; Expressed in embryonic/larval hemocyte (Drosophila) and 22 other tissues.
DR Genevisible; Q9W032; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0070990; F:snRNP binding; IPI:FlyBase.
DR GO; GO:0008362; P:chitin-based embryonic cuticle biosynthetic process; IMP:FlyBase.
DR GO; GO:0006697; P:ecdysone biosynthetic process; TAS:FlyBase.
DR GO; GO:0007281; P:germ cell development; IMP:FlyBase.
DR GO; GO:0007390; P:germ-band shortening; IMP:FlyBase.
DR GO; GO:0007030; P:Golgi organization; IMP:FlyBase.
DR GO; GO:0008258; P:head involution; IMP:FlyBase.
DR GO; GO:0002168; P:instar larval development; IMP:FlyBase.
DR GO; GO:0002165; P:instar larval or pupal development; TAS:FlyBase.
DR GO; GO:0048542; P:lymph gland development; IMP:FlyBase.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0007591; P:molting cycle, chitin-based cuticle; IMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:FlyBase.
DR GO; GO:0016319; P:mushroom body development; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:1903220; P:positive regulation of malate dehydrogenase (decarboxylating) (NADP+) activity; IMP:FlyBase.
DR GO; GO:0007458; P:progression of morphogenetic furrow involved in compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0009608; P:response to symbiont; IMP:FlyBase.
DR InterPro; IPR010770; Ecd.
DR PANTHER; PTHR13060; PTHR13060; 1.
DR Pfam; PF07093; SGT1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; Oogenesis;
KW Reference proteome.
FT CHAIN 1..684
FT /note="Protein ecdysoneless"
FT /id="PRO_0000220846"
FT REGION 491..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 603..624
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 656
FT /note="P->S: Temperature-sensitive mutation; disrupts
FT production of the steroid hormone ecdysone, causes
FT developmental and reproductive defects."
FT /evidence="ECO:0000269|PubMed:15128659"
SQ SEQUENCE 684 AA; 77908 MW; D59B5C943E352F30 CRC64;
MSKIPGSNLE FVREEDFVEY YIFPKIPDNV QDEGALRKLM LQIRNEISAI VREKSLERSY
LWHKDEFQLQ VRLGGAEERL LNEETNPEEE AELGDLPPHF HGVTHYGDNI SDEWFVVYLL
TEITRARGDC IARVSDSDGE FLLIEAADAL PDWASPETCE QRVYLVGGHL QLLQNSAASS
QDKPLTMAMA VQRIRMNPTL YRCSQEIQSC IDARLKEYQI AQPHFSIHRQ VLELPHSAAQ
LLKQKPRLLS SAVRAFCERD SLDIKALRTM RYFPPEATRV RTNVRFTRCL YAMLSHQQYL
PEKRLGWHLT DPVSEPERYK EQLLGLKLAS GLEILATQAK RVEGQQLEDL PAWRSYLRSL
LSKGYFRDNI EGSAEYQELL NKAKVYFRGN QERFRTASRA GAEILDLLLH PAEAASEELR
DEENNLQPSD SDEWLNISAE DLDSMLQDRY GPKKLYKPNG QMNAEEFTKQ LAEFLDRQSN
YEGIEHRGLE EPELDSDDDE PPPQANGSTG LTAKVKKNPS MRKACQRNSV IQPEEPDSTH
VRNFLDFVIP EDNWDSTSEM SDYADEDDME SNLNALSGGG SVFPLDRQIQ SYMEQMDREL
AQTSVGKSFH GKKKTAPQAD EDDFDDIEDF EPININVNTL RNMMDSYQSQ VGGAGPVSNL
FSAMGVGMSA VEDKEQKDIS ESAV
