ECFA1_LACDA
ID ECFA1_LACDA Reviewed; 282 AA.
AC Q1GBJ0;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710};
DE Short=ECF transporter A component EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710};
DE EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN Name=ecfA1 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO1;
GN OrderedLocusNames=Ldb0424;
OS Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081
OS / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM
OS 00102 / Lb 14).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=390333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB
RC 11778 / NCTC 12712 / WDCM 00102 / Lb 14;
RX PubMed=16754859; DOI=10.1073/pnas.0603024103;
RA van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P.,
RA Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R.,
RA Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P.,
RA Weissenbach J., Ehrlich S.D., Maguin E.;
RT "The complete genome sequence of Lactobacillus bulgaricus reveals extensive
RT and ongoing reductive evolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006).
CC -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR EMBL; CR954253; CAI97259.1; -; Genomic_DNA.
DR RefSeq; WP_011543645.1; NZ_JQAV01000001.1.
DR PDB; 5D3M; X-ray; 3.30 A; A/E=2-280.
DR PDB; 5JSZ; X-ray; 3.00 A; A/E=2-282.
DR PDB; 6FNP; X-ray; 3.40 A; B/F=2-282.
DR PDB; 6ZG3; X-ray; 2.80 A; A/F=2-282.
DR PDB; 7NNT; EM; 3.40 A; A=2-282.
DR PDB; 7NNU; EM; 2.70 A; A=2-282.
DR PDBsum; 5D3M; -.
DR PDBsum; 5JSZ; -.
DR PDBsum; 6FNP; -.
DR PDBsum; 6ZG3; -.
DR PDBsum; 7NNT; -.
DR PDBsum; 7NNU; -.
DR AlphaFoldDB; Q1GBJ0; -.
DR SMR; Q1GBJ0; -.
DR STRING; 390333.Ldb0424; -.
DR EnsemblBacteria; CAI97259; CAI97259; Ldb0424.
DR KEGG; ldb:Ldb0424; -.
DR PATRIC; fig|390333.7.peg.381; -.
DR eggNOG; COG1122; Bacteria.
DR HOGENOM; CLU_000604_1_22_9; -.
DR OMA; RMKDFDA; -.
DR BioCyc; LDEL390333:LDB_RS01805-MON; -.
DR Proteomes; UP000001259; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR030947; EcfA_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR04520; ECF_ATPase_1; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51246; CBIO; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Reference proteome; Translocase; Transport.
FT CHAIN 1..282
FT /note="Energy-coupling factor transporter ATP-binding
FT protein EcfA1"
FT /id="PRO_0000287947"
FT DOMAIN 6..243
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT STRAND 2..11
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 17..19
FT /evidence="ECO:0007829|PDB:6FNP"
FT STRAND 22..31
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 42..44
FT /evidence="ECO:0007829|PDB:7NNT"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 49..53
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:6ZG3"
FT TURN 62..64
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 66..69
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:5JSZ"
FT TURN 76..78
FT /evidence="ECO:0007829|PDB:6ZG3"
FT HELIX 79..83
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:7NNU"
FT TURN 93..96
FT /evidence="ECO:0007829|PDB:5JSZ"
FT STRAND 99..101
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 102..106
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 108..112
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 117..130
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 146..159
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 163..169
FT /evidence="ECO:0007829|PDB:7NNU"
FT TURN 170..173
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 176..193
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 196..200
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 204..208
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 210..217
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 220..225
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 227..230
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 231..233
FT /evidence="ECO:0007829|PDB:5JSZ"
FT HELIX 234..239
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 246..256
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 268..280
FT /evidence="ECO:0007829|PDB:7NNU"
SQ SEQUENCE 282 AA; 30845 MW; 6040646A391FCAAF CRC64;
MSDNIISFDH VTFTYPDSPR PALSDLSFAI ERGSWTALIG HNGSGKSTVS KLINGLLAPD
DLDKSSITVD GVKLGADTVW EVREKVGIVF QNPDNQFVGA TVSDDVAFGL ENRAVPRPEM
LKIVAQAVAD VGMADYADSE PSNLSGGQKQ RVAIAGILAV KPQVIILDES TSMLDPEGKE
QILDLVRKIK EDNNLTVISI THDLEEAAGA DQVLVLDDGQ LLDQGKPEEI FPKVEMLKRI
GLDIPFVYRL KQLLKERGIV LPDEIDDDEK LVQSLWQLNS KM
