ECFA1_LEVBA
ID ECFA1_LEVBA Reviewed; 279 AA.
AC Q03PY5;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710};
DE Short=ECF transporter A component EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710};
DE EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN Name=ecfA1 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO1;
GN OrderedLocusNames=LVIS_1662;
OS Levilactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM
OS 1170 / LMG 11437 / NCIMB 947 / NCTC 947) (Lactobacillus brevis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Levilactobacillus.
OX NCBI_TaxID=387344;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB
RC 947 / NCTC 947;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR EMBL; CP000416; ABJ64737.1; -; Genomic_DNA.
DR RefSeq; WP_011668471.1; NC_008497.1.
DR PDB; 4HUQ; X-ray; 3.00 A; B=1-279.
DR PDB; 4HZU; X-ray; 3.53 A; A=1-279.
DR PDB; 4RFS; X-ray; 3.23 A; B=1-279.
DR PDBsum; 4HUQ; -.
DR PDBsum; 4HZU; -.
DR PDBsum; 4RFS; -.
DR AlphaFoldDB; Q03PY5; -.
DR SMR; Q03PY5; -.
DR DIP; DIP-60219N; -.
DR IntAct; Q03PY5; 4.
DR STRING; 387344.LVIS_1662; -.
DR TCDB; 3.A.1.26.9; the atp-binding cassette (abc) superfamily.
DR TCDB; 3.A.1.28.2; the atp-binding cassette (abc) superfamily.
DR EnsemblBacteria; ABJ64737; ABJ64737; LVIS_1662.
DR GeneID; 66421548; -.
DR KEGG; lbr:LVIS_1662; -.
DR eggNOG; COG1122; Bacteria.
DR HOGENOM; CLU_000604_1_22_9; -.
DR OMA; RMKDFDA; -.
DR OrthoDB; 1713578at2; -.
DR Proteomes; UP000001652; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR030947; EcfA_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR04520; ECF_ATPase_1; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51246; CBIO; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Reference proteome; Translocase; Transport.
FT CHAIN 1..279
FT /note="Energy-coupling factor transporter ATP-binding
FT protein EcfA1"
FT /id="PRO_0000287944"
FT DOMAIN 6..240
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT STRAND 4..11
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 14..17
FT /evidence="ECO:0007829|PDB:4HUQ"
FT TURN 18..21
FT /evidence="ECO:0007829|PDB:4RFS"
FT STRAND 23..30
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:4RFS"
FT HELIX 46..52
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 76..80
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 83..86
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:4RFS"
FT STRAND 96..98
FT /evidence="ECO:0007829|PDB:4RFS"
FT HELIX 99..109
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 114..116
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 117..125
FT /evidence="ECO:0007829|PDB:4HUQ"
FT TURN 126..129
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 131..133
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 144..157
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 160..166
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 168..170
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 173..190
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 193..198
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 201..205
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 208..214
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 217..222
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 224..227
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 228..232
FT /evidence="ECO:0007829|PDB:4RFS"
FT HELIX 234..237
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 243..253
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 265..276
FT /evidence="ECO:0007829|PDB:4HUQ"
SQ SEQUENCE 279 AA; 30527 MW; F448B3ABE4FA0E26 CRC64;
MTENIISVDH LTYQYDENQA PALTDVSFTV HAGEWLAIVG HNGSGKSTLA KSLDGLLPFT
QGSVTVGGIT LTPETVWQVR EQIGMIFQNP DNQFVGATVE DDVAFGLENR QISRDEMVPR
VQAALAQVGM TSFAQREPSS LSGGQKQRVA LAGIVAIAPK ILILDEATSM LDPQGRIEML
AIVRQLRQQQ NLTVISITHD IDEAASADRV LVIDDGRLVD EAVPSQIFER GTQLVEMGLD
LPFTEKLKAA LRQRGITPPT TYQTAAEMEE WLWQSLSNT
