ID   ECFA1_MESFL             Reviewed;         406 AA.
AC   Q6F1W5;
DT   21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN   Name=ecfA1 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO1;
GN   OrderedLocusNames=Mfl152;
OS   Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1)
OS   (Acholeplasma florum).
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae;
OC   Mesoplasma.
OX   NCBI_TaxID=265311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33453 / NBRC 100688 / NCTC 11704 / L1;
RA   Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S.,
RA   Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC       proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC       factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; AE017263; AAT75508.1; -; Genomic_DNA.
DR   RefSeq; WP_011183049.1; NC_006055.1.
DR   RefSeq; YP_053392.1; NC_006055.1.
DR   AlphaFoldDB; Q6F1W5; -.
DR   SMR; Q6F1W5; -.
DR   STRING; 265311.Mfl152; -.
DR   PRIDE; Q6F1W5; -.
DR   EnsemblBacteria; AAT75508; AAT75508; Mfl152.
DR   GeneID; 2898234; -.
DR   KEGG; mfl:Mfl152; -.
DR   PATRIC; fig|265311.5.peg.153; -.
DR   eggNOG; COG1122; Bacteria.
DR   HOGENOM; CLU_000604_18_1_14; -.
DR   OMA; AGMTIIM; -.
DR   Proteomes; UP000006647; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR030947; EcfA_1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR04520; ECF_ATPase_1; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Translocase; Transport.
FT   CHAIN           1..406
FT                   /note="Energy-coupling factor transporter ATP-binding
FT                   protein EcfA1"
FT                   /id="PRO_0000092034"
FT   DOMAIN          139..373
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   BINDING         173..180
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ   SEQUENCE   406 AA;  45853 MW;  73A885A3D82BFEBA CRC64;
     MDSLKKFKTT KLTSQDLNDF KIKLHAKYIE MLEANNNFFK FKEAEKQGKI SKEELVEYKN
     KSIKAKKAFK AICQDKSFSE NLKNITKIYN STTRKNSSVI LEQAKEELIE AKNLKREARD
     SVKDHGRGAQ LTKLDKVAIK VENLKFKYAP DFPYALNDVS FEINDGEYVA VIGHNGSGKS
     TLSKMLIGVL SAQEGHVSIY GNVVTQDNLD QARKFLGIVF QNPDNQFIGS SVEADIAFGL
     ENKRVNPKEM QKIIYDSAKK VGMENFLDKE PLNLSGGQKQ RVAIASALAL NPDILIFDEA
     TSMLDPKGNR EIKEIMVELR DKMKKTIISI THDMDEILNA DKVIVMNGGR MVKIGKPEEV
     LKEKEFLRSI KLEIPFLSLV EEALGNEGIK VKHSQNMDEL VKQLCK