ECFA2_LACDA
ID ECFA2_LACDA Reviewed; 287 AA.
AC Q1GBI9;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE Short=ECF transporter A component EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN Name=ecfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO2;
GN OrderedLocusNames=Ldb0425;
OS Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081
OS / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM
OS 00102 / Lb 14).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=390333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB
RC 11778 / NCTC 12712 / WDCM 00102 / Lb 14;
RX PubMed=16754859; DOI=10.1073/pnas.0603024103;
RA van de Guchte M., Penaud S., Grimaldi C., Barbe V., Bryson K., Nicolas P.,
RA Robert C., Oztas S., Mangenot S., Couloux A., Loux V., Dervyn R., Bossy R.,
RA Bolotin A., Batto J.-M., Walunas T., Gibrat J.-F., Bessieres P.,
RA Weissenbach J., Ehrlich S.D., Maguin E.;
RT "The complete genome sequence of Lactobacillus bulgaricus reveals extensive
RT and ongoing reductive evolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:9274-9279(2006).
CC -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR EMBL; CR954253; CAI97260.1; -; Genomic_DNA.
DR RefSeq; WP_003620869.1; NZ_JQAV01000001.1.
DR PDB; 5D3M; X-ray; 3.30 A; B/F=1-287.
DR PDB; 5JSZ; X-ray; 3.00 A; B/F=1-287.
DR PDB; 6FNP; X-ray; 3.40 A; C/G=1-287.
DR PDB; 6ZG3; X-ray; 2.80 A; B/G=1-287.
DR PDB; 7NNT; EM; 3.40 A; B=1-287.
DR PDB; 7NNU; EM; 2.70 A; B=1-287.
DR PDBsum; 5D3M; -.
DR PDBsum; 5JSZ; -.
DR PDBsum; 6FNP; -.
DR PDBsum; 6ZG3; -.
DR PDBsum; 7NNT; -.
DR PDBsum; 7NNU; -.
DR AlphaFoldDB; Q1GBI9; -.
DR SMR; Q1GBI9; -.
DR STRING; 390333.Ldb0425; -.
DR EnsemblBacteria; CAI97260; CAI97260; Ldb0425.
DR GeneID; 66399417; -.
DR KEGG; ldb:Ldb0425; -.
DR PATRIC; fig|390333.13.peg.367; -.
DR eggNOG; COG1122; Bacteria.
DR HOGENOM; CLU_000604_1_22_9; -.
DR OMA; PKYLFCD; -.
DR BioCyc; LDEL390333:LDB_RS01810-MON; -.
DR Proteomes; UP000001259; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR030946; EcfA2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR04521; ECF_ATPase_2; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51246; CBIO; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell membrane; Hydrolase; Membrane;
KW Nucleotide-binding; Reference proteome; Transport.
FT CHAIN 1..287
FT /note="Energy-coupling factor transporter ATP-binding
FT protein EcfA2"
FT /id="PRO_0000287948"
FT DOMAIN 3..245
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT STRAND 3..10
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 12..14
FT /evidence="ECO:0007829|PDB:5JSZ"
FT STRAND 18..20
FT /evidence="ECO:0007829|PDB:6ZG3"
FT STRAND 23..30
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:6ZG3"
FT HELIX 46..53
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:5D3M"
FT STRAND 77..79
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 80..86
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 87..90
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 94..97
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 103..113
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 118..131
FT /evidence="ECO:0007829|PDB:7NNU"
FT TURN 136..140
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 148..160
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 165..171
FT /evidence="ECO:0007829|PDB:7NNU"
FT TURN 172..175
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 178..193
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 197..201
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 206..211
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 213..219
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 222..227
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 229..232
FT /evidence="ECO:0007829|PDB:7NNU"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:6FNP"
FT HELIX 236..241
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 248..259
FT /evidence="ECO:0007829|PDB:7NNU"
FT HELIX 270..280
FT /evidence="ECO:0007829|PDB:7NNU"
SQ SEQUENCE 287 AA; 31630 MW; 5552B71E77C2341C CRC64;
MAIKFENVSY VYSPGSPLEA IGLDQLNFSL EEGKFIALVG HTGSGKSTLM QHFNALLKPT
SGKIEIAGYT ITPETGNKGL KDLRRKVSLA FQFSEAQLFE NTVLKDVEYG PRNFGFSEDE
AREAALKWLK KVGLKDDLIE HSPFDLSGGQ MRRVALAGVL AYEPEIICLD EPAAGLDPMG
RLEMMQLFKD YQAAGHTVIL VTHNMDDVAD YADDVLALEH GRLIKHASPK EVFKDSEWLQ
KHHLAEPRSA RFAAKLEAAG LKLPGQPLTM PELADAIKQS LKGGEHE
