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ECFA2_LEUMM
ID   ECFA2_LEUMM             Reviewed;         285 AA.
AC   Q03ZL5;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
DE   AltName: Full=ECF transporter A component EcfA';
GN   Name=ecfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO2, ecfA';
GN   OrderedLocusNames=LEUM_0226;
OS   Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / DSM
OS   20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523 / NBRC 100496 / NCIMB
OS   8023 / NCTC 12954 / NRRL B-1118 / 37Y).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=203120;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC   / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
RN   [2]
RP   FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION,
RP   SUBSTRATES, AND EXPRESSION IN E.COLI.
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC   / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX   PubMed=18931129; DOI=10.1128/jb.01208-08;
RA   Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA   Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA   Eitinger T.;
RT   "A novel class of modular transporters for vitamins in prokaryotes.";
RL   J. Bacteriol. 191:42-51(2009).
RN   [3]
RP   FUNCTION AS A TRANSPORT COMPONENT, SUBCELLULAR LOCATION, SUBUNIT, AND
RP   EXPRESSION IN E.COLI.
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC   / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX   PubMed=19717603; DOI=10.1128/jb.00965-09;
RA   Neubauer O., Alfandega A., Schoknecht J., Sternberg U., Pohlmann A.,
RA   Eitinger T.;
RT   "Two essential arginine residues in the T components of energy-coupling
RT   factor transporters.";
RL   J. Bacteriol. 191:6482-6488(2009).
CC   -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates including 5-formyltetrahydrofolate, pantothenate
CC       and riboflavin. Expression of the complex plus FolT in E.coli allows 5-
CC       formyltetrahydrofolate uptake; 5-formyltetrahydrofolate is not taken up
CC       in the absence of FolT or the EcfA1A2T complex. {ECO:0000255|HAMAP-
CC       Rule:MF_01710, ECO:0000269|PubMed:18931129,
CC       ECO:0000269|PubMed:19717603}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex probably composed of 2 membrane-embedded substrate-binding
CC       proteins (S component), 2 ATP-binding proteins (A component) and 2
CC       transmembrane proteins (T component). This complex interacts with a
CC       number of substrate-specific components, including FolT, PanT and RibU
CC       for 5-formyltetrahydrofolate, pantothenate and riboflavin respectively.
CC       {ECO:0000269|PubMed:18931129, ECO:0000269|PubMed:19717603}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:18931129,
CC       ECO:0000305|PubMed:19717603}; Peripheral membrane protein
CC       {ECO:0000305|PubMed:18931129, ECO:0000305|PubMed:19717603}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC       factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; CP000414; ABJ61357.1; -; Genomic_DNA.
DR   RefSeq; WP_011679141.1; NC_008531.1.
DR   AlphaFoldDB; Q03ZL5; -.
DR   SMR; Q03ZL5; -.
DR   STRING; 203120.LEUM_0226; -.
DR   EnsemblBacteria; ABJ61357; ABJ61357; LEUM_0226.
DR   GeneID; 61177295; -.
DR   KEGG; lme:LEUM_0226; -.
DR   eggNOG; COG1122; Bacteria.
DR   HOGENOM; CLU_000604_1_22_9; -.
DR   OMA; PKYLFCD; -.
DR   OrthoDB; 1752365at2; -.
DR   Proteomes; UP000000362; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR030946; EcfA2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR04521; ECF_ATPase_2; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell membrane; Hydrolase; Membrane; Nucleotide-binding;
KW   Transport.
FT   CHAIN           1..285
FT                   /note="Energy-coupling factor transporter ATP-binding
FT                   protein EcfA2"
FT                   /id="PRO_0000287962"
FT   DOMAIN          3..245
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   ACT_SITE        171
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ   SEQUENCE   285 AA;  31232 MW;  ACF03E324E038467 CRC64;
     MAINFEQVNF SYGAGTTLAQ PILHDINVTI PDGQVTAIIG QTGSGKSTFI QHLNGLLKPT
     TGRVVIDDFV LTSDLKEKNL TSLRARVGMV FQFPENQLFA NTVLEDVMYA PINFGYAKAD
     AEFAAKTALK QVNVSEELWD KSPFELSGGQ MRRVAMAGTL ASNPDIIVLD EPAAGLDPKG
     QKELLAIVRG LKEAGKLVVF ISHQMDHVIA VADHVIVMHD GGVVAEGTPV EIFNKDLVWF
     KTVALDLPKA GQFAEQLRQK GHILRHRPLL LTELATMLNE EKRHE
 
 
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