ECFA2_LEVBA
ID ECFA2_LEVBA Reviewed; 290 AA.
AC Q03PY6;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE Short=ECF transporter A component EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN Name=ecfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO2;
GN OrderedLocusNames=LVIS_1661;
OS Levilactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM
OS 1170 / LMG 11437 / NCIMB 947 / NCTC 947) (Lactobacillus brevis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Levilactobacillus.
OX NCBI_TaxID=387344;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB
RC 947 / NCTC 947;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR EMBL; CP000416; ABJ64736.1; -; Genomic_DNA.
DR RefSeq; WP_011668470.1; NC_008497.1.
DR PDB; 4HUQ; X-ray; 3.00 A; A=1-290.
DR PDB; 4HZU; X-ray; 3.53 A; B=1-290.
DR PDB; 4RFS; X-ray; 3.23 A; A=1-290.
DR PDBsum; 4HUQ; -.
DR PDBsum; 4HZU; -.
DR PDBsum; 4RFS; -.
DR AlphaFoldDB; Q03PY6; -.
DR SMR; Q03PY6; -.
DR DIP; DIP-60218N; -.
DR IntAct; Q03PY6; 2.
DR STRING; 387344.LVIS_1661; -.
DR TCDB; 3.A.1.26.9; the atp-binding cassette (abc) superfamily.
DR TCDB; 3.A.1.28.2; the atp-binding cassette (abc) superfamily.
DR PRIDE; Q03PY6; -.
DR EnsemblBacteria; ABJ64736; ABJ64736; LVIS_1661.
DR KEGG; lbr:LVIS_1661; -.
DR PATRIC; fig|387344.15.peg.1574; -.
DR eggNOG; COG1122; Bacteria.
DR HOGENOM; CLU_000604_1_22_9; -.
DR OMA; PKYLFCD; -.
DR OrthoDB; 1752365at2; -.
DR Proteomes; UP000001652; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR030946; EcfA2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR04521; ECF_ATPase_2; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51246; CBIO; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell membrane; Hydrolase; Membrane;
KW Nucleotide-binding; Reference proteome; Transport.
FT CHAIN 1..290
FT /note="Energy-coupling factor transporter ATP-binding
FT protein EcfA2"
FT /id="PRO_0000287943"
FT DOMAIN 3..246
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT STRAND 3..13
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 16..18
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 20..26
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 35..39
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 46..52
FT /evidence="ECO:0007829|PDB:4HUQ"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 77..82
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 87..90
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 94..97
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 103..107
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 109..113
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 118..131
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 136..138
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 148..160
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 165..171
FT /evidence="ECO:0007829|PDB:4HUQ"
FT TURN 172..175
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 178..193
FT /evidence="ECO:0007829|PDB:4HUQ"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 198..202
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 206..212
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 214..220
FT /evidence="ECO:0007829|PDB:4HUQ"
FT STRAND 223..228
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 230..233
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 237..242
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 249..259
FT /evidence="ECO:0007829|PDB:4HUQ"
FT HELIX 271..282
FT /evidence="ECO:0007829|PDB:4HUQ"
SQ SEQUENCE 290 AA; 32144 MW; 3C67CA6CD7B8CC6C CRC64;
MAIAFEHVTY TYQAGTPMAH TALTDVSLTV PDRGYLAIIG HTGSGKSTLI QQLNALLKPT
SGTIKIDEFT ITPETTNAAL KPLRQHVGMV FQFPENQLFE ETVRQDIAFG PKNFGMADAD
ALALADEMLT TVGLDQSYAE RSPFELSGGQ MRRVAIAGVL AMQPKVLVLD EPTAGLDPQG
RQEMMRLFAR LHQEQGLTIV LVTHQMEDVA QYAEQVAVMH EGRLMKFGTP ADVFSNREWL
QDHQLDVPQA AQFARRLRDR GLTFPKQPLT ADQLADYLAQ QWAQRGADHV
