ID   ECFA2_MYCPN             Reviewed;         303 AA.
AC   Q50293;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN   Name=ecfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO2;
GN   OrderedLocusNames=MPN_194; ORFNames=GT9_orf303, MP637;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8604303; DOI=10.1093/nar/24.4.628;
RA   Hilbert H., Himmelreich R., Plagens H., Herrmann R.;
RT   "Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma
RT   pneumoniae comprising the dnaA region, the atp operon and a cluster of
RT   ribosomal protein genes.";
RL   Nucleic Acids Res. 24:628-639(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
CC   -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC       proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC       factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; U34795; AAC43687.1; -; Genomic_DNA.
DR   EMBL; U00089; AAB96285.1; -; Genomic_DNA.
DR   PIR; S62814; S62814.
DR   RefSeq; NP_109882.1; NC_000912.1.
DR   RefSeq; WP_010874551.1; NC_000912.1.
DR   AlphaFoldDB; Q50293; -.
DR   SMR; Q50293; -.
DR   IntAct; Q50293; 1.
DR   STRING; 272634.MPN_194; -.
DR   EnsemblBacteria; AAB96285; AAB96285; MPN_194.
DR   GeneID; 66609158; -.
DR   KEGG; mpn:MPN_194; -.
DR   PATRIC; fig|272634.6.peg.212; -.
DR   HOGENOM; CLU_000604_1_22_14; -.
DR   OMA; QVHKYYG; -.
DR   BioCyc; MPNE272634:G1GJ3-310-MON; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR030946; EcfA2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR04521; ECF_ATPase_2; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Hydrolase; Membrane; Nucleotide-binding;
KW   Reference proteome; Transport.
FT   CHAIN           1..303
FT                   /note="Energy-coupling factor transporter ATP-binding
FT                   protein EcfA2"
FT                   /id="PRO_0000092046"
FT   DOMAIN          17..260
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   BINDING         54..61
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ   SEQUENCE   303 AA;  34357 MW;  CCDED25FB41BAF19 CRC64;
     MKKIVELKNP LRDGEILSVS NLSCFFNEKT VHEVKVIDNF SYTFAKNKVY CIIGDSGSGK
     STLVNHFNGL IKPAYGDIWV KDIYIGQKQR KIKDFKRLRK TISIVFQFPE YQLFKDTVEK
     DIMFGPIALG QSKNEARQKA AYYLEKMGLK YTFLERNPFE LSGGQKRRVA IAGILAIEPE
     VLIFDEPTAG LDPEGEREMM RLIKDAKASG RTVFMITHQM ENVLEVADEV LVLSKGQLVK
     SGDPYEVFMD EAFLAHTTII MPPVIQVIKD LINLNPKFSC LLDFKPRNLD QLADAINNTI
     AHG