ID   ECFA_METMJ              Reviewed;         277 AA.
AC   A3CVD3;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component EcfA {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN   Name=ecfA {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO;
GN   OrderedLocusNames=Memar_1404;
OS   Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=368407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35101 / DSM 1498 / JR1;
RX   PubMed=21304656; DOI=10.4056/sigs.32535;
RA   Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C.,
RA   Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L.,
RA   Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT   "Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981
RT   type strain JR1.";
RL   Stand. Genomic Sci. 1:189-196(2009).
CC   -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC       proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC       factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; CP000562; ABN57333.1; -; Genomic_DNA.
DR   RefSeq; WP_011844244.1; NC_009051.1.
DR   AlphaFoldDB; A3CVD3; -.
DR   SMR; A3CVD3; -.
DR   STRING; 368407.Memar_1404; -.
DR   EnsemblBacteria; ABN57333; ABN57333; Memar_1404.
DR   GeneID; 4847303; -.
DR   KEGG; mem:Memar_1404; -.
DR   eggNOG; arCOG00202; Archaea.
DR   HOGENOM; CLU_000604_1_22_2; -.
DR   OMA; YALELCP; -.
DR   OrthoDB; 92866at2157; -.
DR   Proteomes; UP000002146; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:InterPro.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR005876; Co_trans_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01166; cbiO; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Hydrolase; Membrane; Nucleotide-binding;
KW   Transport.
FT   CHAIN           1..277
FT                   /note="Energy-coupling factor transporter ATP-binding
FT                   protein EcfA"
FT                   /id="PRO_0000288019"
FT   DOMAIN          4..238
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   BINDING         37..44
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ   SEQUENCE   277 AA;  30725 MW;  A3A28FEF93331574 CRC64;
     MHLLETRDLT HIYRGDVHAL EGVNFTAERK SRIAVIGPNG AGKSTLFKHF NGILKPTSGE
     VLVRGEPITK ENVREVRKFV GIVFQNPDDQ IFSPTVEQDI AFGPINLGLD EATVAHRVEE
     ALHLLGIEEL RERVPHHLSG GEKKRVAIAG ILAMEPQVLV LDEPTAGLDP QGVADLVAFV
     NRLPEEYGMT VVFSTHHLDL VAEMADYIYV MDKGRVVGSG TVEEVFTRPE LLTQTRLDVP
     PIPKLIRSLR ENGVAIDMAY TYEDAKKSFL DAYARRA