ID   ECFA_METST              Reviewed;         278 AA.
AC   Q2NHA1;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component EcfA {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=3.6.3.- {ECO:0000255|HAMAP-Rule:MF_01710};
GN   Name=ecfA {ECO:0000255|HAMAP-Rule:MF_01710}; Synonyms=cbiO;
GN   OrderedLocusNames=Msp_0401;
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX   PubMed=16385054; DOI=10.1128/jb.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA   Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT   intestinal archaeon is restricted to methanol and H2 for methane formation
RT   and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: ATP-binding (A) component of a common energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC       proteins (T component). {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01710};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Energy-coupling
CC       factor EcfA family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; CP000102; ABC56802.1; -; Genomic_DNA.
DR   RefSeq; WP_011406002.1; NC_007681.1.
DR   AlphaFoldDB; Q2NHA1; -.
DR   SMR; Q2NHA1; -.
DR   STRING; 339860.Msp_0401; -.
DR   EnsemblBacteria; ABC56802; ABC56802; Msp_0401.
DR   GeneID; 41324975; -.
DR   KEGG; mst:Msp_0401; -.
DR   eggNOG; arCOG00202; Archaea.
DR   HOGENOM; CLU_000604_1_22_2; -.
DR   OMA; RCIPFLE; -.
DR   OrthoDB; 92866at2157; -.
DR   Proteomes; UP000001931; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:InterPro.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR005876; Co_trans_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01166; cbiO; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Hydrolase; Membrane; Nucleotide-binding;
KW   Reference proteome; Transport.
FT   CHAIN           1..278
FT                   /note="Energy-coupling factor transporter ATP-binding
FT                   protein EcfA"
FT                   /id="PRO_0000288021"
FT   DOMAIN          5..240
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   BINDING         38..45
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ   SEQUENCE   278 AA;  30551 MW;  804E48729AC7DBDB CRC64;
     MTTILETKNL VYNYPDGTEA LKGIDFKLEE GEMISLLGHN GAGKSTLFLH FNGIIEPTSG
     SVEIDGETLK YDKKSLLAAR QKVGIVFQNP DDQLFAPTVL EDVAFGPMNM GLSEEEVKTR
     SMDALEKVGM SDYAEKPPHH LSGGQKKRVA IAGILSMKPK VMVLDEPTSG LDPNGASSIM
     QLLYDLNKEG MTIIISTHDV DLVPLYSDDI EVIVDGKIIK SGTCKEIFTD KEVIDEADLR
     LPWIGQLFEK LDKEHNITFG NGYPLTVSDA YDALLTKL