ID   ECFG_RHOPT              Reviewed;         181 AA.
AC   B3Q6P8;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=ECF RNA polymerase sigma factor EcfG {ECO:0000305};
DE   AltName: Full=General stress response factor EcfG {ECO:0000303|PubMed:23524612};
GN   Name=ecfG {ECO:0000303|PubMed:23524612};
GN   OrderedLocusNames=Rpal_4706 {ECO:0000312|EMBL:ACF03197.1};
OS   Rhodopseudomonas palustris (strain TIE-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=395960;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TIE-1;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Emerson D.,
RA   Newman D.K., Roden E., Richardson P.;
RT   "Complete sequence of Rhodopseudomonas palustris TIE-1.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION AS A REGULATOR.
RC   STRAIN=TIE-1;
RX   PubMed=23524612; DOI=10.1128/jb.00186-13;
RA   Kulkarni G., Wu C.H., Newman D.K.;
RT   "The general stress response factor EcfG regulates expression of the C-2
RT   hopanoid methylase HpnP in Rhodopseudomonas palustris TIE-1.";
RL   J. Bacteriol. 195:2490-2498(2013).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released (By similarity). Regulates expression of hpnP under a variety
CC       of stresses, including high temperature, pH stress, and presence of
CC       nonionic osmolytes (PubMed:23524612). {ECO:0000250,
CC       ECO:0000269|PubMed:23524612}.
CC   -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC       interaction with the -10 element in promoter DNA, and plays an
CC       important role in melting the double-stranded DNA and the formation of
CC       the transcription bubble. The sigma-70 factor domain-2 mediates
CC       interaction with the RNA polymerase subunits RpoB and RpoC.
CC       {ECO:0000250|UniProtKB:Q9EZJ8}.
CC   -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC       H) motif that mediates interaction with the -35 element in promoter
CC       DNA. The domain also mediates interaction with the RNA polymerase
CC       subunit RpoA. {ECO:0000250|UniProtKB:Q9EZJ8}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP001096; ACF03197.1; -; Genomic_DNA.
DR   RefSeq; WP_011159760.1; NC_011004.1.
DR   AlphaFoldDB; B3Q6P8; -.
DR   SMR; B3Q6P8; -.
DR   EnsemblBacteria; ACF03197; ACF03197; Rpal_4706.
DR   GeneID; 66895351; -.
DR   KEGG; rpt:Rpal_4706; -.
DR   HOGENOM; CLU_047691_1_4_5; -.
DR   OMA; NWNSFRK; -.
DR   OrthoDB; 1914729at2; -.
DR   BioCyc; RPAL395960:RPAL_RS23290-MON; -.
DR   Proteomes; UP000001725; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0006950; P:response to stress; IEA:UniProt.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR039425; RNA_pol_sigma-70-like.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR43133; PTHR43133; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF08281; Sigma70_r4_2; 1.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS01063; SIGMA70_ECF; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Sigma factor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..181
FT                   /note="ECF RNA polymerase sigma factor EcfG"
FT                   /id="PRO_0000434047"
FT   REGION          15..77
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000305"
FT   REGION          103..155
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   181 AA;  19690 MW;  A16DB06E62E969B3 CRC64;
     MPLTDSLRDD ILAAVPSLRA FAISLSGNAD RADDLVQETL LRALANIDSF QPGSNLPAWL
     FTILRNLFRS DYRKRRREVE DADGSYAKTL KSQPGQTAHL EFEEFRAALD KLPQDQREAL
     ILVGASGFSY EDAAAICGCA VGTIKSRVNR ARSKLSALLY VDGAEDFGPD DTVRAVIGGN
     G