ECFT_BACSU
ID ECFT_BACSU Reviewed; 265 AA.
AC P70972;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 3.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Energy-coupling factor transporter transmembrane protein EcfT;
DE Short=ECF transporter T component EcfT;
GN Name=ecfT; Synonyms=ybaF; OrderedLocusNames=BSU01470;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP SEQUENCE REVISION TO 101 AND 262.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [3]
RP FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX PubMed=18931129; DOI=10.1128/jb.01208-08;
RA Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA Eitinger T.;
RT "A novel class of modular transporters for vitamins in prokaryotes.";
RL J. Bacteriol. 191:42-51(2009).
CC -!- FUNCTION: Transmembrane (T) component of an energy-coupling factor
CC (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC transporter provides the energy necessary to transport a number of
CC different substrates (By similarity). Involved in riboflavin transport.
CC {ECO:0000250, ECO:0000269|PubMed:18931129}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). May be able to interact with more than 1 S
CC component at a time (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Abolishes riboflavin uptake, cell growth less
CC inhibited by roseoflavin, a toxic riboflavin analog.
CC {ECO:0000269|PubMed:18931129}.
CC -!- SIMILARITY: Belongs to the energy-coupling factor EcfT family.
CC {ECO:0000305}.
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DR EMBL; AL009126; CAB11923.2; -; Genomic_DNA.
DR PIR; F69742; F69742.
DR RefSeq; NP_388028.2; NC_000964.3.
DR RefSeq; WP_004399656.1; NZ_JNCM01000029.1.
DR AlphaFoldDB; P70972; -.
DR SMR; P70972; -.
DR STRING; 224308.BSU01470; -.
DR PaxDb; P70972; -.
DR PRIDE; P70972; -.
DR EnsemblBacteria; CAB11923; CAB11923; BSU_01470.
DR GeneID; 938923; -.
DR KEGG; bsu:BSU01470; -.
DR PATRIC; fig|224308.179.peg.151; -.
DR eggNOG; COG0619; Bacteria.
DR InParanoid; P70972; -.
DR OMA; MKAQMSR; -.
DR PhylomeDB; P70972; -.
DR BioCyc; BSUB:BSU01470-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01461; EcfT; 1.
DR InterPro; IPR003339; ABC/ECF_trnsptr_transmembrane.
DR InterPro; IPR024919; EcfT.
DR Pfam; PF02361; CbiQ; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..265
FT /note="Energy-coupling factor transporter transmembrane
FT protein EcfT"
FT /id="PRO_0000359933"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 265 AA; 29784 MW; B2FB68F92ECC2C27 CRC64;
MMDSMIIGKY VPGTSLVHRL DPRTKLITIF LFVCIVFLAN NVQTYALLGL FTIGVVSLTR
VPFSFLMKGL KPIIWIVLFT FLLHILMTHE GPIIFQIGFF KVYEGGLVQG IFISLRFVYL
ILITTLLTLT TTPIEITDGM EQLLNPLKKL KLPVHELALM MSISLRFIPT LMEETDKIMK
AQMARGVDFT SGPVKERVKA IVPLLVPLFV SAFKRAEELA VAMEARGYQG GEGRTKYRKL
VWTGKDTSVI VSLIVLAALL FFLRA
