ID   ECFT_LACP3              Reviewed;         264 AA.
AC   Q035B4;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Energy-coupling factor transporter transmembrane protein EcfT;
DE            Short=ECF transporter T component EcfT;
GN   Name=ecfT; OrderedLocusNames=LSEI_2472;
OS   Lacticaseibacillus paracasei (strain ATCC 334 / BCRC 17002 / CCUG 31169 /
OS   CIP 107868 / KCTC 3260 / NRRL B-441) (Lactobacillus paracasei).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lacticaseibacillus.
OX   NCBI_TaxID=321967;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC   B-441;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
RN   [2]
RP   FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBSTRATES, AND EXPRESSION IN
RP   L.LACTIS.
RC   STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC   B-441;
RX   PubMed=18931129; DOI=10.1128/jb.01208-08;
RA   Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA   Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA   Eitinger T.;
RT   "A novel class of modular transporters for vitamins in prokaryotes.";
RL   J. Bacteriol. 191:42-51(2009).
CC   -!- FUNCTION: Part of a common energy-coupling factor (ECF) ABC-transporter
CC       complex. Unlike classic ABC transporters this ECF transporter provides
CC       the energy necessary to transport a number of different substrates
CC       including 5-formyltetrahydrofolate and thiamine. Expression of the
CC       complex plus FolT or ThiT in Lactococcus lactis subsp. cremoris (strain
CC       NZ9000) allows 5-formyltetrahydrofolate or thiamine uptake
CC       respectively; 5-formyltetrahydrofolate or thiamine are not taken up in
CC       the absence of FolT/ThiT or the EcfA1A2T complex. Deenergized L.lactis
CC       subsp. cremoris (treated with 2-deoxyglucose) do not take up substrate.
CC       {ECO:0000269|PubMed:18931129}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex probably composed of 2 membrane-embedded substrate-binding
CC       proteins (S component), 2 ATP-binding proteins (A component) and 2
CC       transmembrane proteins (T component). This complex interacts with a
CC       number of substrate-specific components, including FolT and ThiT for 5-
CC       formyltetrahydrofolate and thiamine respectively. May be able to
CC       interact with more than 1 S component at a time.
CC       {ECO:0000269|PubMed:18931129}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the energy-coupling factor EcfT family.
CC       {ECO:0000305}.
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DR   EMBL; CP000423; ABJ71208.1; -; Genomic_DNA.
DR   RefSeq; WP_003567501.1; NC_008526.1.
DR   RefSeq; YP_807650.1; NC_008526.1.
DR   AlphaFoldDB; Q035B4; -.
DR   SMR; Q035B4; -.
DR   STRING; 321967.LSEI_2472; -.
DR   EnsemblBacteria; ABJ71208; ABJ71208; LSEI_2472.
DR   GeneID; 61270572; -.
DR   KEGG; lca:LSEI_2472; -.
DR   PATRIC; fig|321967.11.peg.2426; -.
DR   HOGENOM; CLU_056469_2_2_9; -.
DR   OMA; NNIMIGR; -.
DR   Proteomes; UP000001651; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01461; EcfT; 1.
DR   InterPro; IPR003339; ABC/ECF_trnsptr_transmembrane.
DR   InterPro; IPR024919; EcfT.
DR   Pfam; PF02361; CbiQ; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..264
FT                   /note="Energy-coupling factor transporter transmembrane
FT                   protein EcfT"
FT                   /id="PRO_0000408991"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   264 AA;  30165 MW;  D24A7773BA47A67F CRC64;
     MDKLLLGRYI PGDSWVHRLD PRTKLIASFY YIGIVFLANN WQTYLMMFVA TLFMIWLSGI
     KIGFFLKGVR PLIWLILFTV VLQVLFVRGG TVYWHWGWLW ITEFGLINGA FIFVRFVLII
     FMSTLLTLST QPLSLADAVE SLLKPLRVIR VPVTELALVL QIALRFVPTL MDQTTKIMNA
     QRARGVDFGE GNIFQQMKAV VPLLIPLFVS SFTTADELAT AMEARGYQGG DDRTKYRILR
     YHRRDWVAAG GMLVLTGLLL LLRA