ID   ECFT_STRT2              Reviewed;         264 AA.
AC   Q5M245;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Energy-coupling factor transporter transmembrane protein EcfT;
DE            Short=ECF transporter T component EcfT;
GN   Name=ecfT; OrderedLocusNames=stu2007;
OS   Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=264199;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-250 / LMG 18311;
RX   PubMed=15543133; DOI=10.1038/nbt1034;
RA   Bolotin A., Quinquis B., Renault P., Sorokin A., Ehrlich S.D.,
RA   Kulakauskas S., Lapidus A., Goltsman E., Mazur M., Pusch G.D., Fonstein M.,
RA   Overbeek R., Kyprides N., Purnelle B., Prozzi D., Ngui K., Masuy D.,
RA   Hancy F., Burteau S., Boutry M., Delcour J., Goffeau A., Hols P.;
RT   "Complete sequence and comparative genome analysis of the dairy bacterium
RT   Streptococcus thermophilus.";
RL   Nat. Biotechnol. 22:1554-1558(2004).
RN   [2]
RP   FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, AND
RP   EXPRESSION IN E.COLI.
RC   STRAIN=ATCC BAA-250 / LMG 18311;
RX   PubMed=23359690; DOI=10.1073/pnas.1217361110;
RA   Karpowich N.K., Wang D.N.;
RT   "Assembly and mechanism of a group II ECF transporter.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013).
CC   -!- FUNCTION: Transmembrane (T) component of an energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates. Expression of the complex plus RibU in de-
CC       energized E.coli allows riboflavin uptake.
CC       {ECO:0000269|PubMed:23359690}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC       proteins (T component) upon coexpression of the components in E.coli.
CC       May be able to interact with more than 1 S component at a time.
CC       {ECO:0000269|PubMed:23359690}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:23359690};
CC       Multi-pass membrane protein {ECO:0000305|PubMed:23359690}.
CC   -!- SIMILARITY: Belongs to the energy-coupling factor EcfT family.
CC       {ECO:0000305}.
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DR   EMBL; CP000023; AAV61601.1; -; Genomic_DNA.
DR   RefSeq; WP_011226702.1; NC_006448.1.
DR   AlphaFoldDB; Q5M245; -.
DR   SMR; Q5M245; -.
DR   STRING; 264199.stu2007; -.
DR   TCDB; 3.A.1.25.6; the atp-binding cassette (abc) superfamily.
DR   EnsemblBacteria; AAV61601; AAV61601; stu2007.
DR   GeneID; 66899733; -.
DR   KEGG; stl:stu2007; -.
DR   eggNOG; COG0619; Bacteria.
DR   HOGENOM; CLU_056469_2_2_9; -.
DR   OMA; MKAQMSR; -.
DR   Proteomes; UP000001170; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0032217; F:riboflavin transmembrane transporter activity; IGI:UniProtKB.
DR   GO; GO:0032218; P:riboflavin transport; IGI:UniProtKB.
DR   HAMAP; MF_01461; EcfT; 1.
DR   InterPro; IPR003339; ABC/ECF_trnsptr_transmembrane.
DR   InterPro; IPR024919; EcfT.
DR   Pfam; PF02361; CbiQ; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..264
FT                   /note="Energy-coupling factor transporter transmembrane
FT                   protein EcfT"
FT                   /id="PRO_0000422259"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   264 AA;  30024 MW;  6B8780D48364B907 CRC64;
     MDKLIIGRYI VGDSFIHRLD PRSKLLAMLI YIIVIFWANN PVTYAVITLF TLFLVFLSKI
     KLGFFLGGIK PMIWIILFST LFQVFFNTRG NVLWSIGFFK ITEVGLNQGW MIFLRFILII
     SFSTLLTLTT TPLSLSDAVE SLLKPLTIFK VPAHEIGLML SLSLRFVPTL MDDTTRIMNA
     QKARGVDFDE GNIIQKVRSI IPILIPLFAS SFKRADALAI AMEARGYRGS EGRTKYRRLL
     WNCRDTLSII AILALGLILF YLKS