ID   ECFT_THEMA              Reviewed;         271 AA.
AC   Q9X2I1; G4FGR0;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Energy-coupling factor transporter transmembrane protein EcfT;
DE            Short=ECF transporter T component EcfT;
GN   Name=ecfT; OrderedLocusNames=TM_1868;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
RN   [2]
RP   FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, AND
RP   EXPRESSION IN E.COLI.
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=23359690; DOI=10.1073/pnas.1217361110;
RA   Karpowich N.K., Wang D.N.;
RT   "Assembly and mechanism of a group II ECF transporter.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013).
CC   -!- FUNCTION: Transmembrane (T) component of an energy-coupling factor
CC       (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF
CC       transporter provides the energy necessary to transport a number of
CC       different substrates (Probable). Expression of the complex plus RibU in
CC       E.coli allows riboflavin uptake; uptake does not occur in the absence
CC       of RibU or the EcfA1A2T complex. {ECO:0000269|PubMed:23359690,
CC       ECO:0000305}.
CC   -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC       complex composed of 2 membrane-embedded substrate-binding proteins (S
CC       component, RibU, BioY), 2 ATP-binding proteins (A component) and 2
CC       transmembrane proteins (T component) upon coexpression in E.coli. A
CC       stable subcomplex with both A and T components are also isolated. This
CC       complex interacts with at least 2 substrate-specific components, BioY
CC       and RibU. {ECO:0000269|PubMed:23359690}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:23359690}; Multi-pass membrane protein
CC       {ECO:0000305|PubMed:23359690}.
CC   -!- SIMILARITY: Belongs to the energy-coupling factor EcfT family.
CC       {ECO:0000305}.
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DR   EMBL; AE000512; AAD36930.1; -; Genomic_DNA.
DR   PIR; E72202; E72202.
DR   RefSeq; NP_229664.1; NC_000853.1.
DR   RefSeq; WP_004082419.1; NZ_CP011107.1.
DR   AlphaFoldDB; Q9X2I1; -.
DR   SMR; Q9X2I1; -.
DR   STRING; 243274.THEMA_04830; -.
DR   TCDB; 3.A.1.25.5; the atp-binding cassette (abc) superfamily.
DR   DNASU; 897358; -.
DR   EnsemblBacteria; AAD36930; AAD36930; TM_1868.
DR   KEGG; tma:TM1868; -.
DR   eggNOG; COG0619; Bacteria.
DR   InParanoid; Q9X2I1; -.
DR   OMA; NNIMIGR; -.
DR   OrthoDB; 1479665at2; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0032217; F:riboflavin transmembrane transporter activity; IGI:UniProtKB.
DR   GO; GO:0032218; P:riboflavin transport; IGI:UniProtKB.
DR   InterPro; IPR003339; ABC/ECF_trnsptr_transmembrane.
DR   Pfam; PF02361; CbiQ; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..271
FT                   /note="Energy-coupling factor transporter transmembrane
FT                   protein EcfT"
FT                   /id="PRO_0000422260"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   271 AA;  30641 MW;  9C782BE2FD0BF2AB CRC64;
     MRLPTVLIGR YIPVDSIVHR LDPRAKLLGM IFLISAVLIV PNLLFYLVPG LAIFLLMFLS
     RTGFKIYLAG LRSLWFFLVF AVLVQFFSSS EGEKIFWMIT DRAIWSAVYI MLRLVLIILL
     AENFSATTPP LLSARAIESI FSTFGARKIG HEIGMVMTIA MRFVPVLALE ADRILKAQIA
     RGANFERGKF FDRIRALVVI IVPLLISALR KAEELAVAME ARLYTGEPPK TRFKDIKWKP
     MDTLYVLLTA GVLVLVLFGR YFVDGVFQYG S