ID   ECH12_MYCBO             Reviewed;         285 AA.
AC   Q7U004; A0A1R3XYG7; X2BIH9;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Probable enoyl-CoA hydratase echA12;
DE            EC=4.2.1.17;
GN   Name=echA12; OrderedLocusNames=BQ2027_MB1507;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIU00110.1; -; Genomic_DNA.
DR   RefSeq; NP_855159.1; NC_002945.3.
DR   RefSeq; WP_003407504.1; NC_002945.4.
DR   AlphaFoldDB; Q7U004; -.
DR   SMR; Q7U004; -.
DR   EnsemblBacteria; SIU00110; SIU00110; BQ2027_MB1507.
DR   GeneID; 45425450; -.
DR   PATRIC; fig|233413.5.peg.1648; -.
DR   OMA; SLEGHMQ; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..285
FT                   /note="Probable enoyl-CoA hydratase echA12"
FT                   /id="PRO_0000109339"
SQ   SEQUENCE   285 AA;  30565 MW;  498D32EB8BD8DD70 CRC64;
     MPHRCAAQVV AGYRSTVSLV LVEHPRPEIA QITLNRPERM NSMAFDVMVP LKEALAQVSY
     DNSVRVVVLT GAGRGFSSGA DHKSAGVVPH VENLTRPTYA LRSMELLDDV ILMLRRLHQP
     VIAAVNGPAI GGGLCLALAA DIRVASSSAY FRAAGINNGL TASELGLSYL LPRAIGSSRA
     FEIMLTGRDV SAEEAERIGL VSRQVPDEQL LDACYAIAAR MAGFSRPGIE LTKRTLWSGL
     DAASLEAHMQ AEGLGQLFVR LLTANFEEAV AARAEQRAPV FTDDT