ID   ECH12_MYCLE             Reviewed;         294 AA.
AC   P53526;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Probable enoyl-CoA hydratase echA12;
DE            EC=4.2.1.17;
GN   Name=echA12; OrderedLocusNames=ML1241; ORFNames=B1170_C2_224, MLCB1610.01;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; U00010; AAA17070.1; -; Genomic_DNA.
DR   EMBL; AL049913; CAB43147.1; -; Genomic_DNA.
DR   EMBL; AL583921; CAC31622.1; -; Genomic_DNA.
DR   PIR; S72706; S72706.
DR   RefSeq; NP_301896.1; NC_002677.1.
DR   RefSeq; WP_010908217.1; NC_002677.1.
DR   AlphaFoldDB; P53526; -.
DR   SMR; P53526; -.
DR   STRING; 272631.ML1241; -.
DR   EnsemblBacteria; CAC31622; CAC31622; CAC31622.
DR   KEGG; mle:ML1241; -.
DR   PATRIC; fig|272631.5.peg.2284; -.
DR   Leproma; ML1241; -.
DR   eggNOG; COG1024; Bacteria.
DR   HOGENOM; CLU_009834_7_2_11; -.
DR   OMA; SLEGHMQ; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase; Reference proteome.
FT   CHAIN           1..294
FT                   /note="Probable enoyl-CoA hydratase echA12"
FT                   /id="PRO_0000109340"
SQ   SEQUENCE   294 AA;  31491 MW;  0B7502E715A7D692 CRC64;
     MSQTDASCTI AELPYRSVTD LVVLDFPRPE VALITLNRPG RMNSMALDLM KSLKQVLKRI
     TYDHSVRVVV LTGAGRGFCS GADQKFTAPV PQVEGLTQPV RALRAMELLE EVILALRRLH
     QPVIAAINGP AIGGGLCLAL AADVRVASTR AYFRAAGINN GLSASELGLS YLLPRAVGSS
     RAFEIMLSGR DVGAEEAEQI GLVSYRVSDD RLLDTCYSIA ARMATFSRSG TELTKRALWG
     GLDAASLDKH MQSESLAQLF IALHTSNFEE AAAPCTEKRP TVLVDARGCA TSPG