ID   ECH12_MYCTO             Reviewed;         285 AA.
AC   P9WNN6; L0T6R6; O53163;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Probable enoyl-CoA hydratase echA12;
DE            EC=4.2.1.17;
GN   Name=echA12; Synonyms=fadB2; OrderedLocusNames=MT1518;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK45783.1; -; Genomic_DNA.
DR   PIR; C70873; C70873.
DR   RefSeq; WP_003898888.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNN6; -.
DR   SMR; P9WNN6; -.
DR   EnsemblBacteria; AAK45783; AAK45783; MT1518.
DR   KEGG; mtc:MT1518; -.
DR   PATRIC; fig|83331.31.peg.1633; -.
DR   HOGENOM; CLU_009834_7_2_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..285
FT                   /note="Probable enoyl-CoA hydratase echA12"
FT                   /id="PRO_0000427095"
SQ   SEQUENCE   285 AA;  30575 MW;  6542FC79E7164F1C CRC64;
     MPHRCAAQVV AGYRSTVSLV LVEHPRPEIA QITLNRPERM NSMAFDVMVP LKEALAQVSY
     DNSVRVVVLT GAGRGFSPGA DHKSAGVVPH VENLTRPTYA LRSMELLDDV ILMLRRLHQP
     VIAAVNGPAI GGGLCLALAA DIRVASSSAY FRAAGINNGL TASELGLSYL LPRAIGSSRA
     FEIMLTGRDV SAEEAERIGL VSRQVPDEQL LDACYAIAAR MAGFSRPGIE LTKRTLWSGL
     DAASLEAHMQ AEGLGQLFVR LLTANFEEAV AARAEQRAPV FTDDT