ID   ECH12_MYCTU             Reviewed;         285 AA.
AC   P9WNN7; L0T6R6; O53163;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Probable enoyl-CoA hydratase echA12;
DE            EC=4.2.1.17;
GN   Name=echA12; Synonyms=fadB2; OrderedLocusNames=Rv1472; ORFNames=MTV007.19;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP44231.1; -; Genomic_DNA.
DR   PIR; C70873; C70873.
DR   RefSeq; NP_215988.1; NC_000962.3.
DR   RefSeq; WP_003898888.1; NZ_NVQJ01000004.1.
DR   AlphaFoldDB; P9WNN7; -.
DR   SMR; P9WNN7; -.
DR   STRING; 83332.Rv1472; -.
DR   PaxDb; P9WNN7; -.
DR   DNASU; 886547; -.
DR   GeneID; 886547; -.
DR   KEGG; mtu:Rv1472; -.
DR   PATRIC; fig|83332.111.peg.1638; -.
DR   TubercuList; Rv1472; -.
DR   eggNOG; COG1024; Bacteria.
DR   OMA; SLEGHMQ; -.
DR   PhylomeDB; P9WNN7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IBA:GO_Central.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   1: Evidence at protein level;
KW   Fatty acid metabolism; Lipid metabolism; Lyase; Reference proteome.
FT   CHAIN           1..285
FT                   /note="Probable enoyl-CoA hydratase echA12"
FT                   /id="PRO_0000109341"
SQ   SEQUENCE   285 AA;  30575 MW;  6542FC79E7164F1C CRC64;
     MPHRCAAQVV AGYRSTVSLV LVEHPRPEIA QITLNRPERM NSMAFDVMVP LKEALAQVSY
     DNSVRVVVLT GAGRGFSPGA DHKSAGVVPH VENLTRPTYA LRSMELLDDV ILMLRRLHQP
     VIAAVNGPAI GGGLCLALAA DIRVASSSAY FRAAGINNGL TASELGLSYL LPRAIGSSRA
     FEIMLTGRDV SAEEAERIGL VSRQVPDEQL LDACYAIAAR MAGFSRPGIE LTKRTLWSGL
     DAASLEAHMQ AEGLGQLFVR LLTANFEEAV AARAEQRAPV FTDDT