ID   ECH14_MYCTO             Reviewed;         256 AA.
AC   P9WNN4; L0T9S3; O53211; P64018;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Probable enoyl-CoA hydratase echA14;
DE            EC=4.2.1.17;
GN   Name=echA14; OrderedLocusNames=MT2560;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK46864.1; -; Genomic_DNA.
DR   PIR; E70868; E70868.
DR   RefSeq; WP_003412728.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNN4; -.
DR   SMR; P9WNN4; -.
DR   EnsemblBacteria; AAK46864; AAK46864; MT2560.
DR   KEGG; mtc:MT2560; -.
DR   PATRIC; fig|83331.31.peg.2762; -.
DR   HOGENOM; CLU_009834_7_2_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..256
FT                   /note="Probable enoyl-CoA hydratase echA14"
FT                   /id="PRO_0000427096"
FT   REGION          235..256
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   256 AA;  26280 MW;  EB314ABF51113AED CRC64;
     MAQYDPVLLS VDKHVALITV NDPDRRNAVT DEMSAQLRAA IQRAEGDPDV HAVVVTGAGK
     AFCAGADLSA LGAGVGDPAE PRLLRLYDGF MAVSSCNLPT IAAVNGAAVG AGLNLALAAD
     VRIAGPAALF DARFQKLGLH PGGGATWMLQ RAVGPQVARA ALLFGMCFDA ESAVRHGLAL
     MVADDPVTAA LELAAGPAAA PREVVLASKA TMRATASPGS LDLEQHELAK RLELGPQAKS
     VQSPEFAARL AAAQHR