ID   ECH17_MYCPA             Reviewed;         257 AA.
AC   Q73VC7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Probable enoyl-CoA hydratase echA17;
DE            EC=4.2.1.17;
GN   Name=echA17; OrderedLocusNames=MAP_3087c;
OS   Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
OS   (Mycobacterium paratuberculosis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=262316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-968 / K-10;
RX   PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA   Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N.,
RA   Kanjilal S., Kapur V.;
RT   "The complete genome sequence of Mycobacterium avium subspecies
RT   paratuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AE016958; AAS05635.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q73VC7; -.
DR   SMR; Q73VC7; -.
DR   STRING; 262316.MAP_3087c; -.
DR   EnsemblBacteria; AAS05635; AAS05635; MAP_3087c.
DR   KEGG; mpa:MAP_3087c; -.
DR   eggNOG; COG1024; Bacteria.
DR   HOGENOM; CLU_009834_7_6_11; -.
DR   OMA; AMEMIMT; -.
DR   Proteomes; UP000000580; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase; Reference proteome.
FT   CHAIN           1..257
FT                   /note="Probable enoyl-CoA hydratase echA17"
FT                   /id="PRO_0000383564"
FT   SITE            139
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   257 AA;  26738 MW;  94E4E9835DFA1699 CRC64;
     MAALNEFVSV VVSDGSRDAG LAMLLVSRPP TNALSRQVYR EVIAAADELG RRDDVAAVIL
     FGGHEIFSAG DDMPELRTLR GAEAETAARV RRDAIDAVAA IPKPTVAAIT GYALGAGLTL
     ALAADWRISG DNVKFGATEI LAGLVPGGDA LARLTRVAGA SKAKELVFSG RFFDAEEALA
     LGLIDEMVAP DDVYDAAAAW ARRFLDGPRH ALAAAKAGVD AVFELPRAER LAAEQRRYVE
     VFSAGQGGDA GADPHGG