ID   ECH17_MYCTA             Reviewed;         261 AA.
AC   A5U753;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Probable enoyl-CoA hydratase EchA17;
DE            EC=4.2.1.17;
GN   Name=echA17; OrderedLocusNames=MRA_3071;
OS   Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=419947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25177 / H37Ra;
RX   PubMed=18584054; DOI=10.1371/journal.pone.0002375;
RA   Zheng H., Lu L., Wang B., Pu S., Zhang X., Zhu G., Shi W., Zhang L.,
RA   Wang H., Wang S., Zhao G., Zhang Y.;
RT   "Genetic basis of virulence attenuation revealed by comparative genomic
RT   analysis of Mycobacterium tuberculosis strain H37Ra versus H37Rv.";
RL   PLoS ONE 3:E2375-E2375(2008).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABQ74853.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000250|UniProtKB:P9WNN3};
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DR   EMBL; CP000611; ABQ74853.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003415942.1; NZ_CP016972.1.
DR   AlphaFoldDB; A5U753; -.
DR   SMR; A5U753; -.
DR   STRING; 419947.MRA_3071; -.
DR   EnsemblBacteria; ABQ74853; ABQ74853; MRA_3071.
DR   GeneID; 45427032; -.
DR   KEGG; mra:MRA_3071; -.
DR   eggNOG; COG1024; Bacteria.
DR   HOGENOM; CLU_009834_7_6_11; -.
DR   OMA; AMEMIMT; -.
DR   OrthoDB; 1498685at2; -.
DR   Proteomes; UP000001988; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..261
FT                   /note="Probable enoyl-CoA hydratase EchA17"
FT                   /id="PRO_0000383566"
FT   SITE            143
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   261 AA;  27178 MW;  40BF00216BEF9DC1 CRC64;
     MAAVTPTVPE FVNVVVSDGS QDAGLAMLLL SRPPTNAMTR QVYREVVAAA NELGRRDDVA
     AVILYGGHEI FSAGDDMPEL RTLSAQEADT AARIRQQAVD AVAAIPKPTV AAITGYALGA
     GLTLALAADW RVSGDNVKFG ATEILAGLIP SGDGMARLTR AAGPSRAKEL VFSGRFFDAE
     EALALGLIDD MVAPDDVYDA AAAWARRFLD GPPHALAAAK AGISDVYELA PAERIAAERR
     RYVEVFAAGQ GGGSKGDRGG R