ID   ECH17_MYCTO             Reviewed;         261 AA.
AC   P9WNN2; L0TEA7; O53286; Q7D686;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 2.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Probable enoyl-CoA hydratase EchA17;
DE            EC=4.2.1.17;
GN   Name=echA17; OrderedLocusNames=MT3124;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK47454.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000250|UniProtKB:P9WNN3};
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DR   EMBL; AE000516; AAK47454.1; ALT_INIT; Genomic_DNA.
DR   PIR; B70860; B70860.
DR   RefSeq; WP_003415942.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNN2; -.
DR   SMR; P9WNN2; -.
DR   EnsemblBacteria; AAK47454; AAK47454; MT3124.
DR   GeneID; 45427032; -.
DR   KEGG; mtc:MT3124; -.
DR   PATRIC; fig|83331.31.peg.3366; -.
DR   HOGENOM; CLU_009834_7_6_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..261
FT                   /note="Probable enoyl-CoA hydratase EchA17"
FT                   /id="PRO_0000427097"
FT   SITE            143
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   261 AA;  27178 MW;  40BF00216BEF9DC1 CRC64;
     MAAVTPTVPE FVNVVVSDGS QDAGLAMLLL SRPPTNAMTR QVYREVVAAA NELGRRDDVA
     AVILYGGHEI FSAGDDMPEL RTLSAQEADT AARIRQQAVD AVAAIPKPTV AAITGYALGA
     GLTLALAADW RVSGDNVKFG ATEILAGLIP SGDGMARLTR AAGPSRAKEL VFSGRFFDAE
     EALALGLIDD MVAPDDVYDA AAAWARRFLD GPPHALAAAK AGISDVYELA PAERIAAERR
     RYVEVFAAGQ GGGSKGDRGG R