ECH20_MYCTU
ID ECH20_MYCTU Reviewed; 247 AA.
AC I6Y3U6;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=(7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-CoA hydrolase {ECO:0000305};
DE Short=HIEC-CoA hydrolase {ECO:0000305};
DE EC=4.1.99.- {ECO:0000250|UniProtKB:Q0S7P8};
GN Name=echA20 {ECO:0000303|PubMed:28377529};
GN OrderedLocusNames=Rv3550 {ECO:0000312|EMBL:CCP46372.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [3]
RP FUNCTION, AND PATHWAY.
RC STRAIN=Erdman;
RX PubMed=28377529; DOI=10.1128/mbio.00321-17;
RA Crowe A.M., Casabon I., Brown K.L., Liu J., Lian J., Rogalski J.C.,
RA Hurst T.E., Snieckus V., Foster L.J., Eltis L.D.;
RT "Catabolism of the last two steroid rings in Mycobacterium tuberculosis and
RT other bacteria.";
RL MBio 8:e00321-e00321(2017).
CC -!- FUNCTION: Involved in the final steps of cholesterol and steroid
CC degradation (PubMed:28377529). Catalyzes the hydrolytic ring D opening
CC of (7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-
CC CoA (HIEC-CoA) to (3E)-2-(2-carboxylatoethyl)-3-methyl-6-oxocyclohex-1-
CC ene-1-carboxyl-CoA (COCHEA-CoA) (By similarity).
CC {ECO:0000250|UniProtKB:Q0S7P8, ECO:0000269|PubMed:28377529}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-indene-
CC carboxyl-CoA + H2O = (3E)-2-(2-carboxylatoethyl)-3-methyl-6-
CC oxocyclohex-1-ene-1-carboxyl-CoA + H(+); Xref=Rhea:RHEA:66360,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:167100,
CC ChEBI:CHEBI:167101; Evidence={ECO:0000250|UniProtKB:Q0S7P8};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66361;
CC Evidence={ECO:0000250|UniProtKB:Q0S7P8};
CC -!- PATHWAY: Steroid metabolism; cholesterol degradation.
CC {ECO:0000269|PubMed:28377529}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP46372.1; -; Genomic_DNA.
DR RefSeq; NP_218067.1; NC_000962.3.
DR RefSeq; WP_003419315.1; NZ_NVQJ01000014.1.
DR AlphaFoldDB; I6Y3U6; -.
DR SMR; I6Y3U6; -.
DR STRING; 83332.Rv3550; -.
DR PaxDb; I6Y3U6; -.
DR DNASU; 888232; -.
DR GeneID; 888232; -.
DR KEGG; mtu:Rv3550; -.
DR PATRIC; fig|83332.111.peg.3955; -.
DR TubercuList; Rv3550; -.
DR eggNOG; COG1024; Bacteria.
DR OMA; CAGVDIK; -.
DR PhylomeDB; I6Y3U6; -.
DR BioCyc; MetaCyc:G185E-7827-MON; -.
DR UniPathway; UPA01058; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IBA:GO_Central.
DR GO; GO:0006707; P:cholesterol catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
PE 1: Evidence at protein level;
KW Cholesterol metabolism; Lipid metabolism; Lyase; Reference proteome;
KW Steroid metabolism; Sterol metabolism.
FT CHAIN 1..247
FT /note="(7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-
FT indene-carboxyl-CoA hydrolase"
FT /id="PRO_0000452310"
SQ SEQUENCE 247 AA; 26333 MW; B833E1DA520386CE CRC64;
MPITSTTPEP GIVAVTVDYP PVNAIPSKAW FDLADAVTAA GANSDTRAVI LRAEGRGFNA
GVDIKEMQRT EGFTALIDAN RGCFAAFRAV YECAVPVIAA VNGFCVGGGI GLVGNSDVIV
ASEDATFGLP EVERGALGAA THLSRLVPQH LMRRLFFTAA TVDAATLQHF GSVHEVVSRD
QLDEAALRVA RDIAAKDTRV IRAAKEALNF IDVQRVNASY RMEQGFTFEL NLAGVADEHR
DAFVKKS
