ID   ECHA8_MYCLE             Reviewed;         257 AA.
AC   O07137;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Probable enoyl-CoA hydratase echA8;
DE            EC=4.2.1.17;
GN   Name=echA8; OrderedLocusNames=ML2402; ORFNames=MLCB1306.05c;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; Y13803; CAA74130.1; -; Genomic_DNA.
DR   EMBL; AL583925; CAC31918.1; -; Genomic_DNA.
DR   PIR; F87209; F87209.
DR   RefSeq; NP_302555.1; NC_002677.1.
DR   RefSeq; WP_010908875.1; NC_002677.1.
DR   AlphaFoldDB; O07137; -.
DR   SMR; O07137; -.
DR   STRING; 272631.ML2402; -.
DR   EnsemblBacteria; CAC31918; CAC31918; CAC31918.
DR   KEGG; mle:ML2402; -.
DR   PATRIC; fig|272631.5.peg.4621; -.
DR   Leproma; ML2402; -.
DR   eggNOG; COG1024; Bacteria.
DR   HOGENOM; CLU_009834_7_6_11; -.
DR   OMA; MDMCLSA; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase; Reference proteome.
FT   CHAIN           1..257
FT                   /note="Probable enoyl-CoA hydratase echA8"
FT                   /id="PRO_0000109337"
SQ   SEQUENCE   257 AA;  27517 MW;  25B801EB690BCD00 CRC64;
     MKYDTILVDG YQRVGIITLN RPQALNALNS QMMNEITNAA KELDIDPDVG AILITGSPKV
     FAAGADIKEM ASLTFTDAFD ADFFSAWGKL AAVRTPMIAA VAGYALGGGC ELAMMCDLLI
     AADTAKFGQP EIKLGVLPGM GGSQRLTRAI GKAKAMDLIL TGRTIDAAEA ERSGLVSRVV
     LADDLLPEAK AVATTISQMS RSATRMAKEA VNRSFESTLA EGLLHERRLF HSTFVTDDQS
     EGMAAFIEKR APQFTHR