ID   ECHA8_MYCTO             Reviewed;         257 AA.
AC   P9WNN8; L0T5R3; O53418; P64016;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Probable enoyl-CoA hydratase echA8;
DE            EC=4.2.1.17;
GN   Name=echA8; OrderedLocusNames=MT1100;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK45356.1; -; Genomic_DNA.
DR   PIR; D70893; D70893.
DR   RefSeq; WP_003405712.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNN8; -.
DR   SMR; P9WNN8; -.
DR   EnsemblBacteria; AAK45356; AAK45356; MT1100.
DR   GeneID; 45425042; -.
DR   KEGG; mtc:MT1100; -.
DR   PATRIC; fig|83331.31.peg.1185; -.
DR   HOGENOM; CLU_009834_7_6_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..257
FT                   /note="Probable enoyl-CoA hydratase echA8"
FT                   /id="PRO_0000427094"
SQ   SEQUENCE   257 AA;  27273 MW;  C8907716969540AA CRC64;
     MTYETILVER DQRVGIITLN RPQALNALNS QVMNEVTSAA TELDDDPDIG AIIITGSAKA
     FAAGADIKEM ADLTFADAFT ADFFATWGKL AAVRTPTIAA VAGYALGGGC ELAMMCDVLI
     AADTAKFGQP EIKLGVLPGM GGSQRLTRAI GKAKAMDLIL TGRTMDAAEA ERSGLVSRVV
     PADDLLTEAR ATATTISQMS ASAARMAKEA VNRAFESSLS EGLLYERRLF HSAFATEDQS
     EGMAAFIEKR APQFTHR