ECHD3_MOUSE
ID ECHD3_MOUSE Reviewed; 300 AA.
AC Q9D7J9; Q99LV0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Enoyl-CoA hydratase domain-containing protein 3, mitochondrial {ECO:0000305};
DE Flags: Precursor;
GN Name=Echdc3 {ECO:0000312|MGI:MGI:1915106};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-110, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: May play a role in fatty acid biosynthesis and insulin
CC sensitivity. {ECO:0000250|UniProtKB:Q96DC8}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
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DR EMBL; AK009166; BAB26117.1; -; mRNA.
DR EMBL; AL845275; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL928735; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC002214; AAH02214.1; -; mRNA.
DR EMBL; BC054365; AAH54365.1; -; mRNA.
DR CCDS; CCDS15672.1; -.
DR RefSeq; NP_077170.2; NM_024208.4.
DR AlphaFoldDB; Q9D7J9; -.
DR SMR; Q9D7J9; -.
DR BioGRID; 212484; 2.
DR STRING; 10090.ENSMUSP00000037371; -.
DR iPTMnet; Q9D7J9; -.
DR PhosphoSitePlus; Q9D7J9; -.
DR SwissPalm; Q9D7J9; -.
DR jPOST; Q9D7J9; -.
DR MaxQB; Q9D7J9; -.
DR PaxDb; Q9D7J9; -.
DR PeptideAtlas; Q9D7J9; -.
DR PRIDE; Q9D7J9; -.
DR ProteomicsDB; 277627; -.
DR Antibodypedia; 24584; 35 antibodies from 15 providers.
DR Ensembl; ENSMUST00000042658; ENSMUSP00000037371; ENSMUSG00000039063.
DR GeneID; 67856; -.
DR KEGG; mmu:67856; -.
DR UCSC; uc008igi.1; mouse.
DR CTD; 79746; -.
DR MGI; MGI:1915106; Echdc3.
DR VEuPathDB; HostDB:ENSMUSG00000039063; -.
DR eggNOG; KOG1682; Eukaryota.
DR GeneTree; ENSGT00940000157926; -.
DR HOGENOM; CLU_009834_7_3_1; -.
DR InParanoid; Q9D7J9; -.
DR OMA; SCDMVVC; -.
DR OrthoDB; 804458at2759; -.
DR PhylomeDB; Q9D7J9; -.
DR TreeFam; TF313089; -.
DR BioGRID-ORCS; 67856; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q9D7J9; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D7J9; protein.
DR Bgee; ENSMUSG00000039063; Expressed in interventricular septum and 166 other tissues.
DR Genevisible; Q9D7J9; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; ISS:UniProtKB.
DR GO; GO:0016836; F:hydro-lyase activity; IBA:GO_Central.
DR GO; GO:0006631; P:fatty acid metabolic process; ISS:UniProtKB.
DR GO; GO:1900078; P:positive regulation of cellular response to insulin stimulus; ISO:MGI.
DR Gene3D; 1.10.12.10; -; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
PE 1: Evidence at protein level;
KW Fatty acid metabolism; Lipid metabolism; Mitochondrion; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..66
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 67..300
FT /note="Enoyl-CoA hydratase domain-containing protein 3,
FT mitochondrial"
FT /id="PRO_0000333216"
FT REGION 34..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 110
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT CONFLICT 50
FT /note="T -> M (in Ref. 3; AAH02214)"
FT /evidence="ECO:0000305"
FT CONFLICT 227
FT /note="E -> G (in Ref. 3; AAH02214)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 32402 MW; 1C0ADB8F105FCCE9 CRC64;
MAVVAGLRAF GVKWPSWLRR NPWAPLSAGF CSPGSAGPAG SESEPRLTST RQQDGIRNIV
LSNPRRRNAL SLAMLKSLRS DILHEAESED LKVIIISAEG PVFSSGHDLK ELTDAQGRDY
HAEVFQTCSE VMMLIRNHPV PILAMVNGLA TAAGCQLVAS CDIAVASDKS SFATPGVNVG
LFCSTPAVAL GRAVPRKVAL EMLFTGEPIS AQEALRHGLI SKVVPEEQLE AETMRIAKKI
SSLSRSVVAL GKATFYKQLP QDLRTAYFLA SQAMVDNLAL QDGQEGIEAF IQKRKPIWSH
