ID   ECHH_RHIME              Reviewed;         257 AA.
AC   Q52995;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Probable enoyl-CoA hydratase;
DE            EC=4.2.1.17;
GN   Name=fadB1; OrderedLocusNames=R00366; ORFNames=SMc01153;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=7751302; DOI=10.1128/jb.177.10.2892-2900.1995;
RA   Margolin W., Bramhill D., Long S.R.;
RT   "The dnaA gene of Rhizobium meliloti lies within an unusual gene
RT   arrangement.";
RL   J. Bacteriol. 177:2892-2900(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC         ChEBI:CHEBI:58856; EC=4.2.1.17;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC         ChEBI:CHEBI:137480; EC=4.2.1.17;
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; L39265; AAA91095.1; -; Genomic_DNA.
DR   EMBL; AL591688; CAC41803.1; -; Genomic_DNA.
DR   RefSeq; NP_384472.1; NC_003047.1.
DR   RefSeq; WP_010968532.1; NC_003047.1.
DR   AlphaFoldDB; Q52995; -.
DR   SMR; Q52995; -.
DR   STRING; 266834.SMc01153; -.
DR   EnsemblBacteria; CAC41803; CAC41803; SMc01153.
DR   GeneID; 61601845; -.
DR   KEGG; sme:SMc01153; -.
DR   PATRIC; fig|266834.11.peg.1738; -.
DR   eggNOG; COG1024; Bacteria.
DR   HOGENOM; CLU_009834_7_6_5; -.
DR   OMA; ELSMSCD; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase; Reference proteome.
FT   CHAIN           1..257
FT                   /note="Probable enoyl-CoA hydratase"
FT                   /id="PRO_0000109344"
FT   CONFLICT        129
FT                   /note="Q -> L (in Ref. 1; AAA91095)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        196
FT                   /note="I -> T (in Ref. 1; AAA91095)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        220
FT                   /note="A -> T (in Ref. 1; AAA91095)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   257 AA;  27472 MW;  971970C6C6E780A7 CRC64;
     MSYETLLVET QGRVGLITLN RPQALNALNA VLMRELDAAL KAFDADRAVG AIVLAGSEKA
     FAAGADIKEM QGLDFVDGYL ADFLGGWEHV ANARKPMIAA VSGFALGGGC ELAMMCDFII
     ASETAKFGQP EITLGVIPGM GGSQRLTRAV GKAKAMDLIL TGRMMDAAEA ERSGLVSRVV
     APDRLLEEAL GAAEKIASFS LPAAMMAKEA VNRSLELTLA EGLRFERRLF QSLFATEDQK
     EGMAAFVAKR KAEFKHR