ECHM_CAEEL
ID ECHM_CAEEL Reviewed; 288 AA.
AC P34559;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Probable enoyl-CoA hydratase, mitochondrial;
DE EC=4.2.1.17;
GN Name=ech-6; ORFNames=T05G5.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:58856; EC=4.2.1.17;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC ChEBI:CHEBI:137480; EC=4.2.1.17;
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z27079; CAA81594.1; -; Genomic_DNA.
DR PIR; S41006; S41006.
DR RefSeq; NP_499156.1; NM_066755.5.
DR AlphaFoldDB; P34559; -.
DR SMR; P34559; -.
DR BioGRID; 41571; 17.
DR DIP; DIP-26850N; -.
DR IntAct; P34559; 4.
DR STRING; 6239.T05G5.6.1; -.
DR World-2DPAGE; 0011:P34559; -.
DR World-2DPAGE; 0020:P34559; -.
DR EPD; P34559; -.
DR PaxDb; P34559; -.
DR PeptideAtlas; P34559; -.
DR EnsemblMetazoa; T05G5.6.1; T05G5.6.1; WBGene00001155.
DR UCSC; T05G5.6.1; c. elegans.
DR WormBase; T05G5.6; CE00318; WBGene00001155; ech-6.
DR eggNOG; KOG1680; Eukaryota.
DR GeneTree; ENSGT00940000157609; -.
DR HOGENOM; CLU_009834_7_6_1; -.
DR InParanoid; P34559; -.
DR OMA; IFKQTDA; -.
DR OrthoDB; 1221604at2759; -.
DR PhylomeDB; P34559; -.
DR Reactome; R-CEL-70895; Branched-chain amino acid catabolism.
DR Reactome; R-CEL-77310; Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA.
DR Reactome; R-CEL-77346; Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.
DR Reactome; R-CEL-77348; Beta oxidation of octanoyl-CoA to hexanoyl-CoA.
DR Reactome; R-CEL-77350; Beta oxidation of hexanoyl-CoA to butanoyl-CoA.
DR Reactome; R-CEL-77352; Beta oxidation of butanoyl-CoA to acetyl-CoA.
DR SignaLink; P34559; -.
DR UniPathway; UPA00659; -.
DR PRO; PR:P34559; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00001155; Expressed in material anatomical entity and 5 other tissues.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:WormBase.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IBA:GO_Central.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR Gene3D; 1.10.12.10; -; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism; Lipid metabolism; Lyase; Mitochondrion;
KW Reference proteome.
FT CHAIN 1..288
FT /note="Probable enoyl-CoA hydratase, mitochondrial"
FT /id="PRO_0000109262"
FT BINDING 96..99
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 139
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 162
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 288 AA; 31172 MW; B7DFE711455CDCF2 CRC64;
MMRFSSMLVR NAKLCANVNQ MQVAAFSSKA PEMIKIEKVG EKQNVALIKL NRPKALNALC
AQLMTELADA LEVLDTDKSV GAIVITGSER AFAAGADIKE MTNNEFATTF SGSFLSNWTA
VSDVKKPVIA AVNGFALGGG NELAMMCDII YAGEKARFGQ PEINIGTIPG AGGTQRWARA
AGKSFAMEVC LTGNHVTAQE AKEHGIVSKI FPADQVVGEA VKLGEKIADQ SPLIVQMAKE
AVNKAYELTL QEGLHFERRL FHATFATKDR KEGMTAFAEK RKPQWESK
