ECLH_DROME
ID ECLH_DROME Reviewed; 97 AA.
AC Q07892; Q4V3X3; Q9VEJ8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Eclosion hormone;
DE AltName: Full=EH;
DE AltName: Full=Ecdysis activator;
DE Flags: Precursor;
GN Name=Eh; ORFNames=CG5400;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=8344291; DOI=10.1111/j.1432-1033.1993.tb18026.x;
RA Horodyski F.M., Ewer J., Riddiford L.M., Truman J.W.;
RT "Isolation, characterization and expression of the eclosion hormone gene of
RT Drosophila melanogaster.";
RL Eur. J. Biochem. 215:221-228(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Neuropeptide that triggers the performance of ecdysis
CC behaviors at the end of a molt. It triggers adult behavior patterns:
CC larval, pupal and adult ecdysis, and plasticization during the molt.
CC {ECO:0000269|PubMed:8344291}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed in a single pair of brain neurons which
CC extend their processes the entire length of the central nervous system
CC and also to the corpora cardiaca portion of the ring gland. These cells
CC show massive depletion of immunoreactive Eh at ecdysis.
CC {ECO:0000269|PubMed:8344291}.
CC -!- SIMILARITY: Belongs to the insect eclosion hormone family.
CC {ECO:0000305}.
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DR EMBL; X72302; CAA51050.1; -; mRNA.
DR EMBL; X72303; CAA51051.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF55423.1; -; Genomic_DNA.
DR EMBL; BT023233; AAY55649.1; -; mRNA.
DR PIR; S34769; S34769.
DR RefSeq; NP_001262668.1; NM_001275739.1.
DR RefSeq; NP_524386.1; NM_079662.3.
DR AlphaFoldDB; Q07892; -.
DR IntAct; Q07892; 8.
DR STRING; 7227.FBpp0082885; -.
DR PaxDb; Q07892; -.
DR DNASU; 42101; -.
DR EnsemblMetazoa; FBtr0083444; FBpp0082885; FBgn0000564.
DR EnsemblMetazoa; FBtr0334930; FBpp0306950; FBgn0000564.
DR GeneID; 42101; -.
DR KEGG; dme:Dmel_CG5400; -.
DR CTD; 103935; -.
DR FlyBase; FBgn0000564; Eh.
DR VEuPathDB; VectorBase:FBgn0000564; -.
DR eggNOG; ENOG502S767; Eukaryota.
DR HOGENOM; CLU_175797_1_0_1; -.
DR InParanoid; Q07892; -.
DR OMA; IGHYTHK; -.
DR OrthoDB; 1621219at2759; -.
DR PhylomeDB; Q07892; -.
DR BioGRID-ORCS; 42101; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Eh; fly.
DR GenomeRNAi; 42101; -.
DR PRO; PR:Q07892; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000564; Expressed in embryonic central brain neuron (Drosophila) and 6 other tissues.
DR ExpressionAtlas; Q07892; baseline and differential.
DR Genevisible; Q07892; DM.
DR GO; GO:0005615; C:extracellular space; ISM:FlyBase.
DR GO; GO:0008255; F:ecdysis-triggering hormone activity; IEA:InterPro.
DR GO; GO:0008031; F:eclosion hormone activity; ISS:FlyBase.
DR GO; GO:0005184; F:neuropeptide hormone activity; TAS:FlyBase.
DR GO; GO:0018990; P:ecdysis, chitin-based cuticle; IMP:FlyBase.
DR GO; GO:0007562; P:eclosion; TAS:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; TAS:FlyBase.
DR GO; GO:0007563; P:regulation of eclosion; ISS:FlyBase.
DR InterPro; IPR006825; Eclosion.
DR Pfam; PF04736; Eclosion; 1.
DR PIRSF; PIRSF001859; Eclosion; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Hormone; Neuropeptide; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..97
FT /note="Eclosion hormone"
FT /id="PRO_0000021144"
FT DISULFID 48..72
FT /evidence="ECO:0000250"
FT DISULFID 52..68
FT /evidence="ECO:0000250"
FT DISULFID 55..83
FT /evidence="ECO:0000250"
SQ SEQUENCE 97 AA; 10610 MW; 6AF87A75041C532C CRC64;
MNCKPLILCT FVAVAMCLVH FGNALPAISH YTHKRFDSMG GIDFVQVCLN NCVQCKTMLG
DYFQGQTCAL SCLKFKGKAI PDCEDIASIA PFLNALE
