ID   ECLH_MANSE              Reviewed;          88 AA.
AC   P11919;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Eclosion hormone;
DE   AltName: Full=EH;
DE   AltName: Full=Ecdysis activator;
DE   Flags: Precursor;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=2813382; DOI=10.1073/pnas.86.20.8123;
RA   Horodyski F.M., Riddiford L.M., Truman J.W.;
RT   "Isolation and expression of the eclosion hormone gene from the tobacco
RT   hornworm, Manduca sexta.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:8123-8127(1989).
RN   [2]
RP   PROTEIN SEQUENCE OF 27-88.
RX   PubMed=3304284; DOI=10.1016/0006-291x(87)90592-4;
RA   Kataoka H., Troetschler R.G., Kramer S.J., Cesarin B.J., Schooley D.A.;
RT   "Isolation and primary structure of the eclosion hormone of the tobacco
RT   hornworm, Manduca sexta.";
RL   Biochem. Biophys. Res. Commun. 146:746-750(1987).
RN   [3]
RP   PROTEIN SEQUENCE OF 27-88.
RX   PubMed=3609300; DOI=10.1016/0014-5793(87)80263-6;
RA   Marti T., Takio K., Walsh K.A., Terzi G., Truman J.W.;
RT   "Microanalysis of the amino acid sequence of the eclosion hormone from the
RT   tobacco hornworm Manduca sexta.";
RL   FEBS Lett. 219:415-418(1987).
RN   [4]
RP   DISULFIDE BONDS.
RX   PubMed=1634328; DOI=10.1111/j.1399-3011.1992.tb01552.x;
RA   Kataoka H., Li J.P., Lui A.S.T., Kramer S.J., Schooley D.A.;
RT   "Complete structure of eclosion hormone of Manduca sexta. Assignment of
RT   disulfide bond location.";
RL   Int. J. Pept. Protein Res. 39:29-35(1992).
CC   -!- FUNCTION: Neuropeptide that triggers the performance of ecdysis
CC       behaviors at the end of a molt. It triggers adult behavior patterns:
CC       larval, pupal and adult ecdysis, and plasticization during the molt.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the insect eclosion hormone family.
CC       {ECO:0000305}.
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DR   EMBL; M27808; AAA29310.1; -; Genomic_DNA.
DR   EMBL; M26922; AAA29311.1; -; mRNA.
DR   PIR; A33993; A33993.
DR   AlphaFoldDB; P11919; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008255; F:ecdysis-triggering hormone activity; IEA:InterPro.
DR   GO; GO:0018990; P:ecdysis, chitin-based cuticle; IEA:InterPro.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR006825; Eclosion.
DR   Pfam; PF04736; Eclosion; 1.
DR   PIRSF; PIRSF001859; Eclosion; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hormone; Neuropeptide; Secreted;
KW   Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000269|PubMed:3304284,
FT                   ECO:0000269|PubMed:3609300"
FT   CHAIN           27..88
FT                   /note="Eclosion hormone"
FT                   /id="PRO_0000021145"
FT   DISULFID        40..64
FT                   /evidence="ECO:0000269|PubMed:1634328"
FT   DISULFID        44..60
FT                   /evidence="ECO:0000269|PubMed:1634328"
FT   DISULFID        47..75
FT                   /evidence="ECO:0000269|PubMed:1634328"
SQ   SEQUENCE   88 AA;  9675 MW;  E488F48656DC1C4E CRC64;
     MAGKVTVAFF MFAMIAFLAN FGYVECNPAI ATGYDPMEIC IENCAQCKKM LGAWFEGPLC
     AESCIKFKGK LIPECEDFAS IAPFLNKL