ID   ECM12_YEAST             Reviewed;         151 AA.
AC   O13529; D3DKW7;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Protein ECM12;
DE   AltName: Full=Extracellular mutant protein 12;
GN   Name=ECM12; OrderedLocusNames=YHR021W-A;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=9335584; DOI=10.1093/genetics/147.2.435;
RA   Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J.,
RA   Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C.,
RA   Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M.,
RA   Davies J., Klis F.M., Robbins P.W., Bussey H.;
RT   "Large scale identification of genes involved in cell surface biosynthesis
RT   and architecture in Saccharomyces cerevisiae.";
RL   Genetics 147:435-450(1997).
CC   -!- FUNCTION: May be involved in cell wall organization and biogenesis.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; U10399; AAB68882.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06711.1; -; Genomic_DNA.
DR   PIR; S52598; S52598.
DR   RefSeq; NP_011886.1; NM_001181428.1.
DR   AlphaFoldDB; O13529; -.
DR   BioGRID; 36452; 12.
DR   STRING; 4932.YHR021W-A; -.
DR   PaxDb; O13529; -.
DR   EnsemblFungi; YHR021W-A_mRNA; YHR021W-A; YHR021W-A.
DR   GeneID; 856416; -.
DR   KEGG; sce:YHR021W-A; -.
DR   SGD; S000003531; ECM12.
DR   VEuPathDB; FungiDB:YHR021W-A; -.
DR   HOGENOM; CLU_1732526_0_0_1; -.
DR   BioCyc; YEAST:G3O-31245-MON; -.
DR   PRO; PR:O13529; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; O13529; protein.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Cell wall biogenesis/degradation; Glycoprotein; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..151
FT                   /note="Protein ECM12"
FT                   /id="PRO_0000239641"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        132
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        137
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   151 AA;  17435 MW;  8F36494519BE7DF0 CRC64;
     MNRSFHFLIK YIYIHVLLVF YFHIKQQAIM PFFIFFFSSF DGLSFDLRVV AFLAKHVFVG
     VCSPFFVVGF FGSSRVVVTE WLSKLVLPPP PVSITQVFSL SRKRGEFSSG YILIINPYKS
     FLRSLLDFSI FNNTAKNKSS TFTLNLEDVS K