ECM15_YEAST
ID ECM15_YEAST Reviewed; 104 AA.
AC P35195; D6VQ01;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=UPF0045 protein ECM15;
DE AltName: Full=Extracellular mutant protein 15;
GN Name=ECM15; OrderedLocusNames=YBL001C; ORFNames=YBL0105;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091860; DOI=10.1002/yea.320100006;
RA Wolfe K.H., Lohan A.J.E.;
RT "Sequence around the centromere of Saccharomyces cerevisiae chromosome II:
RT similarity of CEN2 to CEN4.";
RL Yeast 10:S41-S46(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
RX PubMed=12866058; DOI=10.1002/prot.10443;
RA Tao X., Khayat R., Christendat D., Savchenko A., Xu X.,
RA Goldsmith-Fischman S., Honig B., Edwards A., Arrowsmith C.H., Tong L.;
RT "Crystal structures of MTH1187 and its yeast ortholog YBL001c.";
RL Proteins 52:478-480(2003).
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1900 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the UPF0045 family. {ECO:0000305}.
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DR EMBL; Z26494; CAA81269.1; -; Genomic_DNA.
DR EMBL; Z35762; CAA84816.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07121.1; -; Genomic_DNA.
DR PIR; S44559; S44559.
DR RefSeq; NP_009554.1; NM_001178241.1.
DR PDB; 1LXJ; X-ray; 1.80 A; A=1-104.
DR PDBsum; 1LXJ; -.
DR AlphaFoldDB; P35195; -.
DR SMR; P35195; -.
DR BioGRID; 32701; 80.
DR DIP; DIP-3874N; -.
DR IntAct; P35195; 2.
DR STRING; 4932.YBL001C; -.
DR MaxQB; P35195; -.
DR PaxDb; P35195; -.
DR PRIDE; P35195; -.
DR EnsemblFungi; YBL001C_mRNA; YBL001C; YBL001C.
DR GeneID; 852285; -.
DR KEGG; sce:YBL001C; -.
DR SGD; S000000097; ECM15.
DR VEuPathDB; FungiDB:YBL001C; -.
DR eggNOG; ENOG502S45I; Eukaryota.
DR HOGENOM; CLU_137479_0_2_1; -.
DR InParanoid; P35195; -.
DR OMA; AYGTNIE; -.
DR BioCyc; YEAST:G3O-28907-MON; -.
DR EvolutionaryTrace; P35195; -.
DR PRO; PR:P35195; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P35195; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR Gene3D; 3.30.70.930; -; 1.
DR InterPro; IPR029756; MTH1187/YkoF-like.
DR InterPro; IPR002767; Thiamine_BP.
DR Pfam; PF01910; Thiamine_BP; 1.
DR SUPFAM; SSF89957; SSF89957; 1.
DR TIGRFAMs; TIGR00106; TIGR00106; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..104
FT /note="UPF0045 protein ECM15"
FT /id="PRO_0000147621"
FT STRAND 5..16
FT /evidence="ECO:0007829|PDB:1LXJ"
FT HELIX 23..34
FT /evidence="ECO:0007829|PDB:1LXJ"
FT STRAND 36..43
FT /evidence="ECO:0007829|PDB:1LXJ"
FT STRAND 46..52
FT /evidence="ECO:0007829|PDB:1LXJ"
FT HELIX 53..69
FT /evidence="ECO:0007829|PDB:1LXJ"
FT STRAND 74..82
FT /evidence="ECO:0007829|PDB:1LXJ"
FT HELIX 91..102
FT /evidence="ECO:0007829|PDB:1LXJ"
SQ SEQUENCE 104 AA; 11518 MW; 27674095731E2CC8 CRC64;
MPKIFCLADV CMVPIGTDSA SISDFVALIE KKIRESPLKS TLHSAGTTIE GPWDDVMGLI
GEIHEYGHEK GYVRVHTDIR VGTRTDKHQT AQDKIDVVLK KISQ
