ECM1_YEAST
ID ECM1_YEAST Reviewed; 212 AA.
AC P39715; D6VPG0; E9P8V4;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Shuttling pre-60S factor ECM1;
DE AltName: Full=Extracellular mutant protein 1;
DE AltName: Full=Protein SIM1;
GN Name=ECM1; Synonyms=SIM1; OrderedLocusNames=YAL059W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA Storms R.K.;
RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 102.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11583615; DOI=10.1016/s1097-2765(01)00342-2;
RA Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D.,
RA Lechner J., Hurt E.;
RT "Identification of a 60S preribosomal particle that is closely linked to
RT nuclear export.";
RL Mol. Cell 8:517-529(2001).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND ASSOCIATION WITH THE PRE-60S PARTICLE
RP AND THE NUCLEOPORE COMPLEX.
RX PubMed=20348449; DOI=10.1261/rna.2012310;
RA Yao Y., Demoinet E., Saveanu C., Lenormand P., Jacquier A.,
RA Fromont-Racine M.;
RT "Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export.";
RL RNA 16:1007-1017(2010).
CC -!- FUNCTION: Pre-ribosomal factor involved in 60S ribosomal protein
CC subunit export from the nucleus. {ECO:0000269|PubMed:11583615,
CC ECO:0000269|PubMed:20348449}.
CC -!- SUBUNIT: Associates with the pre-60S ribosomal particle and the
CC nucleopore complex.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus. Nucleus. Cytoplasm.
CC Note=Shuttles between the nucleus and the cytoplasm and is re-imported
CC by the KAP123 karyopherin.
CC -!- MISCELLANEOUS: Present with 2840 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ECM1 family. {ECO:0000305}.
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DR EMBL; U12980; AAC04975.1; -; Genomic_DNA.
DR EMBL; AY558168; AAS56494.1; -; Genomic_DNA.
DR EMBL; BK006935; DAA06930.2; -; Genomic_DNA.
DR PIR; S51963; S51963.
DR RefSeq; NP_009342.2; NM_001178201.2.
DR AlphaFoldDB; P39715; -.
DR SMR; P39715; -.
DR BioGRID; 31771; 147.
DR DIP; DIP-2465N; -.
DR IntAct; P39715; 22.
DR MINT; P39715; -.
DR STRING; 4932.YAL059W; -.
DR iPTMnet; P39715; -.
DR MaxQB; P39715; -.
DR PaxDb; P39715; -.
DR PRIDE; P39715; -.
DR EnsemblFungi; YAL059W_mRNA; YAL059W; YAL059W.
DR GeneID; 851240; -.
DR KEGG; sce:YAL059W; -.
DR SGD; S000000055; ECM1.
DR VEuPathDB; FungiDB:YAL059W; -.
DR eggNOG; ENOG502S24Y; Eukaryota.
DR HOGENOM; CLU_090725_0_0_1; -.
DR InParanoid; P39715; -.
DR OMA; NTRKAGW; -.
DR BioCyc; YEAST:G3O-28862-MON; -.
DR PRO; PR:P39715; -.
DR Proteomes; UP000002311; Chromosome I.
DR RNAct; P39715; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IDA:SGD.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IGI:SGD.
DR InterPro; IPR022784; Ribosome_bgen_Alb1.
DR Pfam; PF09135; Alb1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW Ribosome biogenesis; Transport.
FT CHAIN 1..212
FT /note="Shuttling pre-60S factor ECM1"
FT /id="PRO_0000086916"
FT REGION 28..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 188..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 102
FT /note="A -> D (in Ref. 1; AAC04975)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 212 AA; 23891 MW; ACFFE6CFF6F62CD5 CRC64;
MWEQRRQKVV FSLTILVRYR LKQSMAKKIS KNSRAARQSD ALEPEVKDLS ELPRAEKTDL
TNILIRTAAK NEALLEAKIS KKANKSKRGK KLNKKALEDK LANSISSMDR DRLVKALNFT
NRLDGKIAKS ISRAKYIQNT RKAGWDSTNE TIKKELAFLN GGLSVQAKSA SEGNAEKEDE
EIPEVFDSLA EDNTVQKTPT NRFGVLPDDV EE
