ECM27_YEAST
ID ECM27_YEAST Reviewed; 725 AA.
AC P47144; D6VWS5;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Protein ECM27;
DE AltName: Full=Extracellular matrix protein 27;
GN Name=ECM27; OrderedLocusNames=YJR106W; ORFNames=J1978;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 219.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION.
RX PubMed=9335584; DOI=10.1093/genetics/147.2.435;
RA Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J.,
RA Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C.,
RA Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M.,
RA Davies J., Klis F.M., Robbins P.W., Bussey H.;
RT "Large scale identification of genes involved in cell surface biosynthesis
RT and architecture in Saccharomyces cerevisiae.";
RL Genetics 147:435-450(1997).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. {ECO:0000305}.
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DR EMBL; Z49606; CAA89636.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08891.2; -; Genomic_DNA.
DR PIR; S57127; S57127.
DR RefSeq; NP_012640.4; NM_001181764.4.
DR AlphaFoldDB; P47144; -.
DR BioGRID; 33862; 46.
DR DIP; DIP-7290N; -.
DR IntAct; P47144; 2.
DR MINT; P47144; -.
DR STRING; 4932.YJR106W; -.
DR iPTMnet; P47144; -.
DR MaxQB; P47144; -.
DR PaxDb; P47144; -.
DR PRIDE; P47144; -.
DR EnsemblFungi; YJR106W_mRNA; YJR106W; YJR106W.
DR GeneID; 853570; -.
DR KEGG; sce:YJR106W; -.
DR SGD; S000003867; ECM27.
DR VEuPathDB; FungiDB:YJR106W; -.
DR eggNOG; KOG2399; Eukaryota.
DR GeneTree; ENSGT00940000157433; -.
DR HOGENOM; CLU_004979_2_1_1; -.
DR InParanoid; P47144; -.
DR OMA; CASDYLC; -.
DR BioCyc; YEAST:G3O-31731-MON; -.
DR Reactome; R-SCE-425561; Sodium/Calcium exchangers.
DR Reactome; R-SCE-8949215; Mitochondrial calcium ion transport.
DR PRO; PR:P47144; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47144; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006812; P:cation transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:SGD.
DR GO; GO:0070316; P:regulation of G0 to G1 transition; IMP:SGD.
DR Gene3D; 1.20.1420.30; -; 2.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..725
FT /note="Protein ECM27"
FT /id="PRO_0000209503"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 526..546
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 621..641
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 668..688
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 704..724
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 219
FT /note="N -> D (in Ref. 1; CAA89636)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 725 AA; 80656 MW; 0C648B818BF9B325 CRC64;
MDWAINVAHP RLLYKDPKLS VTFIVPSLFH IIIAFVLLGI CASDFLCPNV AHISDPNSLR
SNGSLVSKTA SHASHTGALM AVLLSWCNSS PDLFSNLMSW ATSTRETRST SVSLSIGEVL
GACGIILCIV EGSIFIIMSR THIEISQIQK LSIMRDLLFS LAAMCVMSYV SLMNQVTVLN
CLLMAFLYAF YLVVKLTFKL NHSAETPDET AADTSLRENS VSPFLDDSLM ASGLLPPIQP
GFDISNSITH GIKPSLLSAM DFNSFLSMLE NSSLEEDDSR NEMAELNTLR SMTPGQHWSA
SATVAGEATS AGRPFSEPTN AFTEYRDSER AINSSPAVFA PYRDNPDDEE SQEQVLLETT
THGHFGAQEM RRFSKRSLGW IIKIFIPHLS NFSQKSISDA IFSIITVPFF IIFKLSCPQP
PSDILSYDPT LNRYSLTTLP IILLFIQSIT APFLLCSILS VLLTYHLGYL VYLFPLILAM
ALILLLTAFI TKVNLHNKFT LSLDSSNILQ EKLQKRKLLE RLNTSIQIIF LAIGIINIII
WISLLANSLI EMMEIYQKIL GLSKAILGLT IFAWGNSVGD LISNISMCRL YKTQTHYQDR
VRLATKFFMI SCASCLGGVM LNSMGGIGFS GLVSMLFIGA FNDNEWWFLR KVKLQETSQL
DNTLNYKFIV SCVFIILQII LLLLFFGGPN NIKRRLTKEM KLVGISMCGL WALATLINIL
LELFS
