ECM29_DROME
ID ECM29_DROME Reviewed; 1890 AA.
AC Q9V677; Q7K1A6;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Proteasome-associated protein ECM29 homolog;
GN ORFNames=CG8858;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213 AND SER-1683, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1683; THR-1691 AND SER-1692,
RP AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- SUBUNIT: Associated with the proteasome. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL39369.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF58554.1; -; Genomic_DNA.
DR EMBL; AY069224; AAL39369.2; ALT_INIT; mRNA.
DR RefSeq; NP_610746.1; NM_136902.3.
DR AlphaFoldDB; Q9V677; -.
DR BioGRID; 62097; 1.
DR IntAct; Q9V677; 2.
DR STRING; 7227.FBpp0087116; -.
DR iPTMnet; Q9V677; -.
DR PaxDb; Q9V677; -.
DR PRIDE; Q9V677; -.
DR DNASU; 36319; -.
DR EnsemblMetazoa; FBtr0088008; FBpp0087116; FBgn0033698.
DR GeneID; 36319; -.
DR KEGG; dme:Dmel_CG8858; -.
DR UCSC; CG8858-RA; d. melanogaster.
DR FlyBase; FBgn0033698; CG8858.
DR VEuPathDB; VectorBase:FBgn0033698; -.
DR eggNOG; KOG0915; Eukaryota.
DR GeneTree; ENSGT00940000153612; -.
DR HOGENOM; CLU_000880_2_1_1; -.
DR InParanoid; Q9V677; -.
DR OMA; FFAMHEE; -.
DR OrthoDB; 392862at2759; -.
DR PhylomeDB; Q9V677; -.
DR BioGRID-ORCS; 36319; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36319; -.
DR PRO; PR:Q9V677; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033698; Expressed in eye disc (Drosophila) and 25 other tissues.
DR Genevisible; Q9V677; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000502; C:proteasome complex; ISS:UniProtKB.
DR GO; GO:0060090; F:molecular adaptor activity; IBA:GO_Central.
DR GO; GO:0043248; P:proteasome assembly; IEA:InterPro.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR024372; Ecm29.
DR PANTHER; PTHR23346:SF19; PTHR23346:SF19; 1.
DR Pfam; PF13001; Ecm29; 1.
DR SUPFAM; SSF48371; SSF48371; 3.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Proteasome; Reference proteome; Repeat.
FT CHAIN 1..1890
FT /note="Proteasome-associated protein ECM29 homolog"
FT /id="PRO_0000212562"
FT REPEAT 6..29
FT /note="HEAT 1"
FT REPEAT 30..67
FT /note="HEAT 2"
FT REPEAT 130..167
FT /note="HEAT 3"
FT REPEAT 226..263
FT /note="HEAT 4"
FT REPEAT 294..330
FT /note="HEAT 5"
FT REPEAT 334..354
FT /note="HEAT 6"
FT REPEAT 355..395
FT /note="HEAT 7"
FT REPEAT 459..496
FT /note="HEAT 8"
FT REPEAT 498..523
FT /note="HEAT 9"
FT REPEAT 524..561
FT /note="HEAT 10"
FT REPEAT 565..602
FT /note="HEAT 11"
FT REPEAT 685..722
FT /note="HEAT 12"
FT REPEAT 776..813
FT /note="HEAT 13"
FT REPEAT 843..882
FT /note="HEAT 14"
FT REPEAT 938..975
FT /note="HEAT 15"
FT REPEAT 980..1018
FT /note="HEAT 16"
FT REPEAT 1118..1155
FT /note="HEAT 17"
FT REPEAT 1159..1196
FT /note="HEAT 18"
FT REPEAT 1271..1309
FT /note="HEAT 19"
FT REPEAT 1313..1350
FT /note="HEAT 20"
FT REPEAT 1378..1415
FT /note="HEAT 21"
FT REPEAT 1416..1457
FT /note="HEAT 22"
FT REPEAT 1497..1534
FT /note="HEAT 23"
FT REPEAT 1541..1578
FT /note="HEAT 24"
FT REPEAT 1583..1620
FT /note="HEAT 25"
FT REPEAT 1623..1660
FT /note="HEAT 26"
FT REPEAT 1751..1788
FT /note="HEAT 27"
FT REPEAT 1826..1863
FT /note="HEAT 28"
FT REGION 1680..1702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1213
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 1683
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656,
FT ECO:0000269|PubMed:18327897"
FT MOD_RES 1691
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1692
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1890 AA; 212087 MW; 2EBE44AD13A55DBD CRC64;
METGPNAEIE LLERVLLRLG NADSDEKLEA AVGKFLTPVI LKMNSPHNVV RTKVVEVLTH
IKRRLSSRGH VQIPVEALLD QYAAQDSSTF LKNFAIIFIT MGFPRLALEQ QTALASKVVG
CEDKLESYQD KLFALLLPVL SDMKIPDDPT QRSKLLDLQD KPAICANFLA LLQDVLLLPY
GITQEQDVPP GLSPYSFTRI IAYNWRAEEL EKVKKGIVRF LCASVFSDLQ IFVPLVVASA
DTRFSVATPA ITELSKLCTM LDFSNPAVTA PLYTLFAGNQ SKITDRQTRP CCARVRQKLL
QYLIKCRGKS INVTKGLQVT FDGFFGTNTN QKCKVLALQF MELLLRDGPR ELVSKVSKVI
LTGITKIIGR DSNEPNDVQN AAYSALAQHA RSFPQDVSQD LKLVLGYFNN LASCAPELHS
SIREALVSMA PAFAWKTKEK SDAMEVDKNV DSSSVKLDGQ QHLLLAMLLD NAESKVQIVQ
NVTSVFLTSC YPEYYAPARY LLLLIAGERN SLRENVTTYL YGTSKKDHIN YSMLSSIDHS
KNRISSESIS DFNHLSLEQR RVMLPSFQVM MSHVHEMAEK RLKKNTACVV VGRTKLPYSL
EVYEELLDYL RLCLWYSAGV VAAPGDEKYT HELREYITLH YEDTEDNALH QYAQFVQRSV
EAKRSESNLI CLYDLLNAAP ELFAAKQLHL LEPLGNTLKD VSETMRINVA QVYGILWAFG
LSDEKFDEEV GECLNSLTQK TLEHKHGWLL VVGHTFNRKI AMLKQENKTK DFAAWPQFVN
AVKIISKCLC ESQWLLVSAA VKCISMIGKA VEIPNVPVEI QVPSKDGDDD DEEMTEYTSI
DSSTKLVIFG VVFQLLRSSS ARQKIREDSA RCLGYLAIGD GEHFTKRNLD KFLTLTKIQK
DAALNIAISE AIVNTLCGYD VNKGQPDEKF VNKHCNDDDF DQFLNSLIRL VTDPNPHSRQ
AISVWLLAVV KHCSQRPAVL AKKELLQFAF TELLSDDSEF VQDVASRGLG LVYSISDSGS
QSDLANSLLD QLIGGKRKVN QVTADTELFA EGMLGKTPTG GNITTYKELC SLASDLNQPD
MIYQFMQLAN HNATWTSKLG AAFGLKTLSA ESRQKMEPYL GKIIPRLYRY KYDPTPKIQN
SMISIWDTIV TDSKEVTERY YWEILRELLD NLTCKEWRVR IACCLAVRDL LNRPNGLKLR
SEEPVRRALP DNSMEVDEVP EPELKELWFQ LFRVMDDIHE GTRVAAHGTA SFLGKLCVIA
SSSDHGKSGT AVASSILPFL LETGVGHKVP EIRRVSIKTI SDMIDSSGSL IAPHLATLIP
CLLRATGELE NTKLSYVSTR LGADNEAQEA VDTLRAEAAK SLHTMETIGK CVRYIDYSVL
EKMTPEVLEL MKGSVNLGTK IGCAHFVCLI SIRLGKEMTP LVGKYIRACF VGIKNRNATV
RKYNASAIGH LLGLAKEQSI KSLFTKLEEL YAEQPGNRSI ALTIQSINKR HHELLKDYMD
SMLPLIFFAM HEEPNEETKA NVELWKDLWH DVSPGDAGIR LNLNVIIPKL ESSLTDASWS
RKAQAANAIQ TIATRLSSSL DEPDRLRLIK LLLSGLQGRT FEGKERLLQA LAALTKGLDR
NHQICSSIID AAMREARKRE PVYRTMALAS LGEILDQLEA DRFEEVYNMS WNLLEKKELR
KESDDEDEPN TSQELSADER NKRAQTLNRL KEVVWETLGK SWPRHSIETQ HRYQLFFAEN
CTSILAESTR PVQVSLLAAL TKYIERLHVF DESAQVPDLT QINQEKKIKT EETAPKTREA
IVEKICTDVL AAVALAAGVP HSGLKKEALN IVLMLIKRLS GSGNGNQQPL RLIKQSFESN
LEKFQRDSAP EIRCRIKDIE DKLSKLNPQN
