ECM7_YEAST
ID ECM7_YEAST Reviewed; 448 AA.
AC Q06200; D6VZ78; Q2VQX1;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Protein ECM7;
DE AltName: Full=Extracellular mutant protein 7;
DE AltName: Full=Zinc-regulated gene 15 protein;
GN Name=ECM7; Synonyms=ZRG15; OrderedLocusNames=YLR443W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-95.
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=15905473; DOI=10.1093/nar/gki583;
RA Zhang Z., Dietrich F.S.;
RT "Mapping of transcription start sites in Saccharomyces cerevisiae using 5'
RT SAGE.";
RL Nucleic Acids Res. 33:2838-2851(2005).
RN [4]
RP IDENTIFICATION.
RX PubMed=9335584; DOI=10.1093/genetics/147.2.435;
RA Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J.,
RA Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C.,
RA Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M.,
RA Davies J., Klis F.M., Robbins P.W., Bussey H.;
RT "Large scale identification of genes involved in cell surface biosynthesis
RT and architecture in Saccharomyces cerevisiae.";
RL Genetics 147:435-450(1997).
RN [5]
RP IDENTIFICATION.
RX PubMed=10978274; DOI=10.1093/genetics/156.1.45;
RA Yuan D.S.;
RT "Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon
RT tagging.";
RL Genetics 156:45-58(2000).
RN [6]
RP MEMBRANE TOPOLOGY.
RX PubMed=12524434; DOI=10.1074/jbc.m300163200;
RA Kim H., Melen K., von Heijne G.;
RT "Topology models for 37 Saccharomyces cerevisiae membrane proteins based on
RT C-terminal reporter fusions and predictions.";
RL J. Biol. Chem. 278:10208-10213(2003).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: May be involved in cell wall organization and biogenesis.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Repressed by zinc.
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DR EMBL; U22382; AAB67532.1; -; Genomic_DNA.
DR EMBL; AY899247; AAX83932.1; -; mRNA.
DR EMBL; BK006945; DAA09744.1; -; Genomic_DNA.
DR PIR; S55966; S55966.
DR RefSeq; NP_013548.1; NM_001182331.1.
DR AlphaFoldDB; Q06200; -.
DR BioGRID; 31702; 98.
DR DIP; DIP-5013N; -.
DR IntAct; Q06200; 1.
DR STRING; 4932.YLR443W; -.
DR TCDB; 1.H.1.4.6; the claudin tight junction (claudin1) family.
DR iPTMnet; Q06200; -.
DR MaxQB; Q06200; -.
DR PaxDb; Q06200; -.
DR PRIDE; Q06200; -.
DR EnsemblFungi; YLR443W_mRNA; YLR443W; YLR443W.
DR GeneID; 851164; -.
DR KEGG; sce:YLR443W; -.
DR SGD; S000004435; ECM7.
DR VEuPathDB; FungiDB:YLR443W; -.
DR eggNOG; ENOG502R7IE; Eukaryota.
DR HOGENOM; CLU_042615_0_0_1; -.
DR InParanoid; Q06200; -.
DR OMA; WSGLEWC; -.
DR BioCyc; YEAST:G3O-32499-MON; -.
DR PRO; PR:Q06200; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06200; protein.
DR GO; GO:0051285; C:cell cortex of cell tip; IBA:GO_Central.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; IMP:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR InterPro; IPR009571; SUR7/Rim9-like_fungi.
DR Pfam; PF06687; SUR7; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Zinc.
FT CHAIN 1..448
FT /note="Protein ECM7"
FT /id="PRO_0000086923"
FT TOPO_DOM 1..28
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..204
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..246
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..287
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 351..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 351..408
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 448 AA; 50328 MW; 736CE44DF3CDAE97 CRC64;
MVMSRIRDTI ARPFQNLTAL EKVVQWLRLG TTLLIISFGL ALTVGPLSSP RTLYMSRLDT
YSADITTGLF TVLRESMEQS TSTEENNGVG LTTSELYILT AYTESQIKNV PQYITVSLYG
RCDSTYTMVE VFDSEGNMHS VKNSTTKSTC SSIGTDYLFD YREVLESLGL DIILDYAYNK
IGSQQAESSA YTTYMRSLKH KKANVLHLLY AVISFQVCML FFMIWYYYIK GRFMNALKER
ALVHINSLLS LVVFIGGLIS SISLAWVNYT IQSRINTELE AFGFSYHLGV TWFALLWCFA
GLISVSCLAW SGLEWCISDN GTSYGGGIDD KFLGYQAGVF TDADLDDETS YSQRYPQRQS
TSGEAELMRN SDTMATIRKT SDVDLNSEND ANTSLDHGNP TANISNGGKH EPFATREEFE
LQDIRFRSSN DSEESMQRVI KPSSALQF
