ID   ECOT_YERPY              Reviewed;         169 AA.
AC   B1JSA0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Ecotin {ECO:0000255|HAMAP-Rule:MF_00706};
DE   Flags: Precursor;
GN   Name=eco {ECO:0000255|HAMAP-Rule:MF_00706}; OrderedLocusNames=YPK_2851;
OS   Yersinia pseudotuberculosis serotype O:3 (strain YPIII).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=502800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YPIII;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C.,
RA   Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.;
RT   "Complete sequence of Yersinia pseudotuberculosis YPIII.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: General inhibitor of pancreatic serine proteases: inhibits
CC       chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a
CC       variety of other proteases. {ECO:0000255|HAMAP-Rule:MF_00706}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00706}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_00706}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I11 (ecotin) family.
CC       {ECO:0000255|HAMAP-Rule:MF_00706}.
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DR   EMBL; CP000950; ACA69127.1; -; Genomic_DNA.
DR   RefSeq; WP_002210815.1; NZ_CP009792.1.
DR   PDB; 2Y6T; X-ray; 2.74 A; E/F/G/H=22-169.
DR   PDBsum; 2Y6T; -.
DR   AlphaFoldDB; B1JSA0; -.
DR   SMR; B1JSA0; -.
DR   MEROPS; I11.001; -.
DR   EnsemblBacteria; ACA69127; ACA69127; YPK_2851.
DR   GeneID; 66842315; -.
DR   KEGG; ypy:YPK_2851; -.
DR   PATRIC; fig|502800.11.peg.3566; -.
DR   OMA; PKAEKGM; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd00242; Ecotin; 1.
DR   HAMAP; MF_00706; Ecotin; 1.
DR   InterPro; IPR036198; Ecotin_sf.
DR   InterPro; IPR005658; Prot_inh_ecotin.
DR   InterPro; IPR023084; Prot_inh_ecotin_gammaproteobac.
DR   PANTHER; PTHR35890; PTHR35890; 1.
DR   Pfam; PF03974; Ecotin; 1.
DR   PIRSF; PIRSF006865; Prot_inh_ecotin; 1.
DR   SUPFAM; SSF49772; SSF49772; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Periplasm; Protease inhibitor;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00706"
FT   CHAIN           22..169
FT                   /note="Ecotin"
FT                   /id="PRO_5000316227"
FT   SITE            110..111
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00706"
FT   DISULFID        76..113
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00706"
FT   HELIX           33..35
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          44..51
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   HELIX           59..61
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          62..74
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          79..82
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          85..89
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   TURN            91..93
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          96..100
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          107..109
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          120..127
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   TURN            129..132
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          133..135
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          142..147
FT                   /evidence="ECO:0007829|PDB:2Y6T"
FT   STRAND          151..159
FT                   /evidence="ECO:0007829|PDB:2Y6T"
SQ   SEQUENCE   169 AA;  18871 MW;  EFD86EFBB7F6FF16 CRC64;
     MKKCSIILAS VLLATSINAI ADTPTPLNQQ QPLEKIAPYP QAEKGMSRQV IFLEPQKDES
     RFKVELLIGK TLNVDCNRHM LGGNLETRTL SGWGFDYLVM DKISQPASTM MACPEDSKPQ
     VKFVTANLGD AAMQRYNSRL PIVVYVPQGV EVKYRIWEAG EDIRSAQVK