ID   ECP44_DAUCA             Reviewed;         258 AA.
AC   Q9XJ56;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Phosphoprotein ECPP44;
GN   Name=ECPP44;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039 {ECO:0000312|EMBL:BAA82445.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. US-Harumakigosun {ECO:0000269|Ref.1};
RC   TISSUE=Embryo {ECO:0000269|Ref.1};
RA   Tan S.-K., Sage-Ono K., Kamada H.;
RT   "Cloning and characterization of ECPP44, a cDNA encoding a 44-kilodalton
RT   phosphoprotein relating to somatic embryogenesis in carrot.";
RL   Plant Biotechnol. 17:61-68(2000).
RN   [2] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 59-179, PROTEIN SEQUENCE OF 59-79; 98-117 AND
RP   161-179, INDUCTION, AND PHOSPHORYLATION.
RC   STRAIN=cv. US-Harumakigosun {ECO:0000269|Ref.2};
RC   TISSUE=Embryo {ECO:0000269|Ref.2};
RA   Tan S.-K., Kamada H.;
RT   "Initial identification of a phosphoprotein that appears to be involved in
RT   the induction of somatic embryogenesis in carrot.";
RL   Plant Cell Rep. 19:739-747(2000).
CC   -!- FUNCTION: Phosphorylation of ECCP44 protein is thought to be involved
CC       in the acquisition of embryogenic competence. Unlike other dehydrins,
CC       it is not thought to function as an environmental stress tolerant.
CC       {ECO:0000303|Ref.2}.
CC   -!- INDUCTION: By stress compounds such as sucrose, sodium chloride,
CC       calcium chloride and abscisic acid in shoot apices.
CC       {ECO:0000269|Ref.2}.
CC   -!- PTM: Phosphorylated in embryogenic and somatic embryos. Not
CC       phosphorylated in non-embryogenic cells. {ECO:0000269|Ref.2}.
CC   -!- SIMILARITY: Belongs to the plant dehydrin family. {ECO:0000305}.
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DR   EMBL; AB010898; BAA82445.1; -; mRNA.
DR   AlphaFoldDB; Q9XJ56; -.
DR   SMR; Q9XJ56; -.
DR   GO; GO:0009414; P:response to water deprivation; IEA:UniProt.
DR   InterPro; IPR000167; Dehydrin.
DR   InterPro; IPR030513; Dehydrin_CS.
DR   PANTHER; PTHR33346; PTHR33346; 1.
DR   Pfam; PF00257; Dehydrin; 2.
DR   PROSITE; PS00315; DEHYDRIN_1; 1.
DR   PROSITE; PS00823; DEHYDRIN_2; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Phosphoprotein; Stress response.
FT   CHAIN           1..258
FT                   /note="Phosphoprotein ECPP44"
FT                   /id="PRO_0000100044"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          46..131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          148..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..25
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        46..76
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        94..131
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   258 AA;  28795 MW;  6E9DB45BEF2D7575 CRC64;
     MASDDSVPQH SVEKTTEYES SDRGLFDFMK KEEKDETKVI ATEFEEKVQV SEPEPKYEDC
     KVVEEEEEKA AKPSLLEKLH RSGSSSSSSS SDEEVEEGGE KKKKKEKKGL KEKIEEKIHH
     KEEDTSVPVE VVTEPEKKKG FMEKIKEKLP GGGKKVEEET VAPPPPPAAA PVDCAVEGDP
     AKKGILEKIK EKIPGYHPKT STEEEKKDND CASAKLIIRC LDRMFDYYYY ASFSCGVGLI
     LCFDPLLWGP LISFGTSG