ID   ECPA_ECO57              Reviewed;         195 AA.
AC   P0AAA4; P77264;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Common pilus major fimbrillin subunit EcpA;
DE   AltName: Full=MatB fimbrillin;
DE   Flags: Precursor;
GN   Name=ecpA; Synonyms=matB, yagZ; OrderedLocusNames=Z0360, ECs0323;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND GENE NAME.
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=17563352; DOI=10.1073/pnas.0704104104;
RA   Rendon M.A., Saldana Z., Erdem A.L., Monteiro-Neto V., Vazquez A.,
RA   Kaper J.B., Puente J.L., Giron J.A.;
RT   "Commensal and pathogenic Escherichia coli use a common pilus adherence
RT   factor for epithelial cell colonization.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:10637-10642(2007).
RN   [4]
RP   INDUCTION.
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=22797761; DOI=10.1128/jb.00915-12;
RA   Martinez-Santos V.I., Medrano-Lopez A., Saldana Z., Giron J.A.,
RA   Puente J.L.;
RT   "Transcriptional regulation of the ecp operon by EcpR, IHF, and H-NS in
RT   attaching and effacing Escherichia coli.";
RL   J. Bacteriol. 194:5020-5033(2012).
CC   -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli common
CC       pilus (ECP). ECP is found in both commensal and pathogenic strains and
CC       plays a dual role in early-stage biofilm development and host cell
CC       recognition. Major subunit of the fimbria.
CC       {ECO:0000269|PubMed:17563352}.
CC   -!- SUBUNIT: Self-associates. Forms filaments. Interacts with EcpD (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000250}.
CC   -!- INDUCTION: Negatively regulated by H-NS. Positively regulated by IHF
CC       and EcpR. {ECO:0000269|PubMed:22797761}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant lacks ECP production and is
CC       impaired in adherence compared with the wild-type strain.
CC       {ECO:0000269|PubMed:17563352}.
CC   -!- SIMILARITY: Belongs to the EcpA/MatB fimbrillin family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG54618.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33746.1; -; Genomic_DNA.
DR   PIR; C90669; C90669.
DR   PIR; F85519; F85519.
DR   RefSeq; NP_308350.1; NC_002695.1.
DR   RefSeq; WP_000730972.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AAA4; -.
DR   SMR; P0AAA4; -.
DR   STRING; 155864.EDL933_0324; -.
DR   EnsemblBacteria; AAG54618; AAG54618; Z0360.
DR   EnsemblBacteria; BAB33746; BAB33746; ECs_0323.
DR   GeneID; 914422; -.
DR   KEGG; ece:Z0360; -.
DR   KEGG; ecs:ECs_0323; -.
DR   PATRIC; fig|386585.9.peg.417; -.
DR   eggNOG; ENOG502Z9JQ; Bacteria.
DR   HOGENOM; CLU_120328_0_0_6; -.
DR   OMA; AQDQFTF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.3290; -; 1.
DR   InterPro; IPR016514; EcpA.
DR   InterPro; IPR038478; Fimbrillin_EcpA_sf.
DR   Pfam; PF16449; MatB; 1.
DR   PIRSF; PIRSF007320; Fimbrillin_MatB; 1.
PE   2: Evidence at transcript level;
KW   Fimbrium; Reference proteome; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..195
FT                   /note="Common pilus major fimbrillin subunit EcpA"
FT                   /id="PRO_0000041956"
SQ   SEQUENCE   195 AA;  20111 MW;  60791615380A2A44 CRC64;
     MKKKVLAIAL VTVFTGMGVA QAADVTAQAV ATWSATAKKD TTSKLVVTPL GSLAFQYAEG
     IKGFNSQKGL FDVAIEGDST ATAFKLTSRL ITNTLTQLDT SGSTLNVGVD YNGAAVEKTG
     DTVMIDTANG VLGGNLSPLA NGYNASNRTT AQDGFTFSII SGTTNGTTAV TDYSTLPEGI
     WSGDVSVQFD ATWTS